THYMULIN EIA ELISA KIT
INTENDED USE
The Thymulin EIA Kit Assay is intended for the quantitative determination of Thymulin in serum and thymus preparations. It is for research use only.
SUMMARY AND EXPLANATION OF TEST
The thymus regulates multiple functions. It is mainly responsible for the immune reactions. In addition, it influences the central nervous system and the endocrinium by the secretion of single peptides like Thymulin and Thymosines. Thymulin is only active as a zinc complex, and acts on Tlymphocytes and their precursor stem cells. The secretion of Thymulin is regulated by the pituitary gland.
PRINCIPLE OF THE TEST
Thymulin EIA Kit
This Thymulin EIA Kit can be used for the determination of Thymulin in serum and thymus preparations. The Thymulin EIA Kit test principle is based on a competition between the antigen in the sample or standards and biotinylated thymulin as a tracer for the binding sites of anti-Thymulin antibodies coated on the wells of the microplate. A peroxidase-conjugated steptavidin is used for detection and quantification, and tetramethylbenzidine (TMB) as a peroxidase substrate. The enzymatic reaction is terminated by an acidic stop solution. A dose response curve of the absorbance unit (optical density, OD at 450 nm) vs. concentration is generated using the values obtained from the standards. Thymulin present in the patient samples is determined directly from this curve.
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