ALANINE AMINOTRANSFERASE (ALT, SGPT) REAGENT (UV-KINETIC)
INTENDED USE
Alanine Aminotransferase (ALT, SGPT) Reagent (UV-Kinetic) can be used for the quantitative determination of alanine aminotransferase in human serum.
INTRODUCTION
The enzyme alanine aminotransferase is widely reported in a variety of tissue sources. The major source of ALT is of hepatic origin and has led to the application of ALT determinations in the study of hepatic diseases. Elevated serum levels are found in hepatitis, cirrhosis, and obstructive jaundice. Levels of ALT are only slightly elevated in patients following a myocardial infarction. UV methods for ALT determination were first developed by Wroblewski and LaDue in 1956. The method was based on the oxidation of NADH by lactate dehydrogenase (LDH). In 1980, the International Federation of Clinical Chemistry recommended a reference procedure for the measurements of ALT based on the Wroblewski and LaDue procedure. The ALT reagent conforms to the formulation recommended by the IFCC.
PRINCIPLE
Alanine Aminotransferase (ALT, SGPT) Reagent (UV-Kinetic)
The pyruvate formed in the first reaction is reduced to lactate in the presence of lactate dehydrogenase and NADH. The activity of ALT is determined by measuring the rate of oxidation of NADH at 340 nm. Endogenous sample pyruvate is converted to lactate by LDH during the lag phase prior to measurement.
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