CREATINE KINASE-MB (CK-MB) REAGENT SET
INTENDED USE
Creatine Kinase-MB (CK-MB) Reagent Set is intended to measure the activity of isoenzyme CK-MB in human serum.
INTRODUCTION
Creatine kinases are dimeric molecules composed of M and B subunits and exist as the isoenzymes MM, MB, and BB. The subunits M and B are immunologically distinct; CK-MM and CK-MB are distributed primarily in the skeletal muscle and heart muscle, respectively. While CK-BB is present mainly in the brain and in tissues composed of smooth muscle.
Following acute myocardial infarction, CK-MB activity increases significantly and this elevation is highly specific for the laboratory diagnosis of myocardial infarction. Although total CK activity usually increases following myocardial infarction, in some patients only the CK-MB activity increases, while the total CK remains in the normal range. In conventional methods, CK isoenzymes are quantitated after first separating the three species by electrophoresis, column anion exchange, or batch anion exchange chromatography. However, these methods are time consuming. In recent times, Wurzburg et al. has introduced an immunoinhibition method. This methodology forms the basis of our CK-MB reagent.
PRINCIPLE
Creatine Kinase-MB (CK-MB) Reagent Set
The sample is incubated in the CK-MB reagent which includes the anti-CK-M antibody. CK-B catalyzes the reversible phosphorylation of ADP, in the presence of creatine phosphate, to form ATP and creatine. The auxiliary enzyme hexokinase (HK) catalyzes the phosphorylation of glucose by the ATP format, to produce ADP and glucose-6-phosphate (G-6-P) is oxidized to 6-phosphogluconate with the concomitant production of NADH. The rate of NADH formation, measured at 340 nm, is directly proportional to serum CK-B activity.
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