GAMMA-GLUTAMYL TRANSFERASE REAGENT SET (KINETIC)(SOLUBLE SUBSTRATE)
INTENDED USE
Gamma Glutamyl Transferase Reagent Set (Kinetic) can be used for the quantitative determination of g-GT in human serum.
INTRODUCTION
Gamma-glutamyl transferase (g-GT) is one of a large group of enzymes known as peptidases. A peptidase is an enzyme that catalyzes the hydrolytic cleavage of peptides to form amino acids or smaller peptides. g-GT catalyzes the transfer of g-glutamyl groups from peptides or peptide like compounds to an acceptor peptide molecule. g-GT was originally termed a "transpeptidase" but the nomenclature was later changed to the more appropriate term "transferase".
Although renal tissue has the highest level of g-GT, the major source of the enzyme present in serum is of hepatic origin. Elevated levels of g-GT are found in association with hepatobiliary, pancreatic disorders, alcoholics, myocardial disorders, and diabetics.
Various methods have been employed for the assay of g-GT activity. Glutathione was used for assessing g-GT activity in the early time. A synthetic substrate g-glutamyl-p-nitroanilide (GGPNA) has been used more extensively. Glycylglycine was introduced as an activator and Szasz adapted this activator and the GGPNA to a 30°C kinetic photometric method.3 Rosalki adapted optimal concentrations of the activator and GGPNA substrate to a 37°C colorimetric endpoint method. Our procedure has been optimized according to Szasz and Rosalki and is performed as either an endpoint or kinetic procedure.
PRINCIPLE
Gamma Glutamyl Transferase Reagent Set (Kinetic)
g-GT catalyzes the transfer of a g-glutamyl group from GGPNA. The rate of liberation of p-nitroaniline is directly related to the g-GT activity in the sample and is quantitated by measuring the increase in absorbance at 405 nm.
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