MutaGEL® PARODONTITS PCR KIT
INTENDED USE
The MutaGEL® Parodontits PCR kit allows the detection of a selection of up to three from the five major pathogens (Porphyromonas gingivalis, Tannerella forsythensis, Fusobacterium nucleatum, Actinobacillus actinomycetemcomitans and Prevotella intermedia) leading to chronic parodontitis, agressive juvenile parodontitis and necrotic ulcerative gingivitis. Matrix for this analysis is bacteria-DNA isolated from gingival pocket.
INTRODUCTION
Parodontitis is the main cause of teeth-lost in adults. The mucous membranes of healthy human cavity of the mouth are colonized with billions of microorganisms. The different species (about 300) secrete growth factors and inhibitors thereby regulating their own number. This ecological system of microorganisms is in permanent steady state. Beside the physiological bacteria also pathogen organisms will grow up in the mouth (more precise in the gingival pocket). An excessive amplification of these pathogens disturbs the physiological ballance and favours development of parodontitis (making a therapy with antibiotics necessary). Once started, parodontitis leads in its further course to slow degradation of the parodontium. This stage begins mostly at the age of 30-35 years resulting in first teeth losts at the age of 40 years (if no treatment occurs).
Diagnostic questions are A) chronic parodontitits (Porphyromonas gingivalis, Tannerellaforsythensis, Fusobacterium necrotum), B) aggressive parodontitis in younger patients (Actinobacillus actinomycetemcomitans) and C) necrotic ulcerative ginigivitis (Prevotella intermedia).
PRINCIPLE OF THE TEST
MutaGEL® Parodontitis PCR kit
The MutaGEL® Parodontitis PCR kit contains different sets of primer which amplify each a specific sequence of the following pathogens: Porphyromonas gingivalis, Tannerella forsythensis, Fusobacterium nucleatum, Actinobacillus actinomycetemcomitans and Prevotella intermedia. Additionally, withprimers for a highly conserved region of bacterial 16S-RNA gene, an optical quantification of all detected pathogens can be done (analogous to theclassical determination of germ numbers). In dependence of the diagnostic question a set (up to 3) of the required primers must be choosen.Subsequently, the single pathogens are analysed in parallel and all generated amplicons have different sizes allowing a separation by classical gelelectrophoreticmethods.
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