ANTI-GLIADINS IgA ELISA KIT
INTENDED USE
The Anti-Gliadins IgA ELISA Kit Assay is intended for the quantitative determination of anti-gliadins IgA antibodies in stool. For in vitro diagnostic use only.
INTRODUCTION
The celiac/coeliac disease is caused by the gliadin fraction of wheat gluten and similar proteins of rye and barley. The disease is manly manifested as chronic digestion insufficiency in children or young adults. In addition, patients with celiac disease have a greatly increased risk of developing malignant T-cell lymphoma of the small bowel, as T-cell lymphoma was found in 6 of 10 patients with coeliac sprue. Early diagnosis of celiac disease is important, because there is evidence that a gluten-free diet might help to prevent complications and malignancies (including intestinal lymphoma).
The measurement of anti-gliadin-sIgA-antibodies may be useful as a screening criterion before jejunal biopsy, the "Gold Standard“, and for the monitoring of the gluten-free diet treatment.
PRINCIPLE OF THE TEST
Anti-Gliadins IgA ELISA Kit
Anti-Gliadins IgA ELISA Kit Assay is intended for the quantitative determination of anti-gliadins IgA antibodies in stool. The antigen gliadin is immobilized on the microtiter plate. During the first incubation step, the human anti-gliadin-sIgA antibodies in the samples are bound by the immobilized antigen. After a washing step, the anti-gliadinsIgA antibodies of the samples are quantitatively determined by addition of a peroxidase-labeled anti-gliadin-sIgA antibody mix. Tetramethylbenzidine (TMB) is used as a peroxidase substrate. The enzymatic reaction is stopped by an acidic stop solution. The absorbance of the color compound is determined photometrically at 450 nm. The measured absorbance is directly proportional to the amount of bound anti-gliadin-sIgA antibodies.
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