ANTI-HUMAN TISSUE TRANSGLUTAMINASE (htTG) sIgA ELISA KIT
INTENDED USE
The Anti-Human tissue Transglutaminase (htTG) sIgA ELISA Kit Assay is intended for the quantitative determination of anti-human tissue Transglutaminase sIgA antibodies in stool. For in vitro diagnostic use only.
INTRODUCTION
Dieterich et al. have shown, that tissue transglutaminase (tTG) is the predominant endomysial auto antigen characteristic for celiac disease.
In most celiac patients, usually several disorders are observed, e.g. malabsorption, infertility, osteoporosis and delayed growth of children. But it has also been widely reported that celiac disease is associated with a whole series of autoimmune diseases like Dermatitis herpetiformis Duhring, Diabetes mellitus, rheumatoid Arthritis, IgA-nephritis, neuro-psychiatric disorders, Hashimoto-Thyreoditis / M. Basedow and an increased risk of developing malignant T cell lymphoma.
Because the prevalence of associated autoimmune diseases in most cases is high, it is advisable to determine the auto antibodies against tissue transglutaminase (tTG) as a marker for celiac disease.
PRINCIPLE OF THE TEST
Anti-Human tissue Transglutaminase (htTG) sIgA ELISA Kit
The Anti-Human tissue Transglutaminase (htTG) sIgA ELISA Kit is used for semiquantitative determination of anti-htTG sIgA antibodies in faeces. In a first incubation step, the anti-htTG sIgA antibodies in the sample are bound to their antigen (human, recombinant Transglutaminase), which is immobilized to the surface of the microtiter plates. To remove all unbound foreign substances, a washing step is carried out. In a second incubation step, a POD-labeled sIgA antibody mix is added. After another washing step, to remove all unbound antibodies, the solid phase is incubated with the substrate, tetramethylbenzidine (TMB). An acidic stop solution is then added to stop the reaction. The color converts from blue to yellow. The intensity of the yellow color is directly proportional to the amount of bound antibodies and can be determined photometrically at 450 nm.
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