APRIL ELISA KIT
INTENDED USE
The APRIL ELISA Kit is intended for the quantitative determination of APRIL (A Proliferation-Inducing Ligand) in cell culture supernatant. It is for research use only.
INTRODUCTION
APRIL - a type II transmembrane protein [250 aa, cytoplasmatic domain: 28aa, transmembrane domain: 21aa, extracellular domain: 201aa] – is considered to be involved in autoimmune and cancer diseases. It stimulates proliferation of tumor cell lines and seems to play a role in tumorgenesis. In general it is involved in B and T cell proliferation, triggers humoral immune responses, activates NF-κB and induces cell death.
TACI (transmembrane activator) and BCMA (B-cell maturation antigen) are considered to be receptors for APRIL. APRIL belongs to the TNF-Superfamily which is involved in immune
responses and apoptosis.
PRINCIPLE OF THE TEST
APRIL ELISA Kit
APRIL ELISA Kit is intended for the quantitative determination of APRIL (A Proliferation-Inducing Ligand) in cell culture supernatant. This APRIL ELISA Kit assay utilizes the two-site “sandwich” technique with two selected antibodies (polyclonal and monoclonal) that bind to human APRIL.
APRIL ELISA Kit Assay standards and samples to be tested for human APRIL are added to wells of microplate that was coated with an anti-human APRIL antibody. After the first incubation period, antibodies immobilized on the wall of microtiter wells capture human APRIL in the samples. A detection antibody (monoclonal anti-APRIL antibody) is added to each well. In an incubation step, the detection antibody binds to APRIL. Then a peroxidase-conjugated antibody is added to each microtiter well and a “sandwich” of a capture antibody - human APRIL – detection antibody - Peroxidase conjugate is formed. Tetramethylbenzidine (TMB) is used as a substrate for the peroxidase. Finally, an acidic stop solution is added to terminate the reaction. The color changes from blue to yellow. The intensity of the yellow color is directly proportional to the concentration of APRIL. A dose response curve of the absorbance unit (optical density, OD at 450 nm) vs. concentration is generated, using the values obtained from the standards. APRIL present in the samples, is determined directly from this curve.
Download Product Data Sheet