ANTIBODIES AGAINST TNFa BLOCKER (ADA) ELISA KIT
INTENDED USE
The Antibodies Against TNFa Blocker (ADA) ELISA Kit is intended for the qualitative determination of free human antibodies against therapeutic anti-TNFa antibodies in the rheumatism therapy (e.g. antibodies against HUMIRA) in serum. It is for research use only.
CLINICAL RELEVANCE
With our ELISA test, free antibodies against therapeutic antibodies (anti-TNFa therapeutic antibodies, e.g. HUMIRA) in the rheumatism therapy can be detected. Anti-TNFa therapeutic antibodies are used for suppressing therapy in rheumatic patients. There is a possibility that patients under anti-TNFa-antibody therapy develop antibodies against the therapeutic antibodies. This might lead to severe complications, even to a systemic anaphylaxis with possibly lethal outcome.
The Antibodies Against TNFa Blocker (ADA) ELISA Kit can be used for therapy monitoring and offers the doctor a tool for decision on possible preventive measures.
PRINCIPLE OF THE TEST
Antibodies Against TNFa Blocker (ADA) ELISA Kit
Antibodies Against TNFa Blocker (ADA) ELISA Kit is intended for the qualitative determination of free human antibodies against therapeutic anti-TNFa antibodies in the rheumatism therapy (e.g. antibodies against HUMIRA) in serum. This Antibodies Against TNFa Blocker (ADA) ELISA Kit Assay is a sandwich assay for the determination of free antibodies against therapeutic antibodies (anti-TNFa therapeutic antibodies, e.g. HUMIRA) in serum samples. In a first incubation step, the free anti-therapeutic antibodies from the sample are bound to the therapeutic antibodies (e.g. HUMIRA) coated on the plate. To remove all unbound substances, a washing step is carried out. In a further incubation step, Peroxidase-labeled therapy antibody is added. After another washing step, to remove all unbound substances, the solid phase is incubated with the substrate, Tetramethylbenzidine (TMB). An acidic stop solution is then added. The colour converts to yellow. The absorbance of the color compound is determined photometrically at 450 nm. The intensity of the color is directly proportional to the amount of bound anti-therapeutic antibodies (e.g. anti- HUMIRA) from the sample. The results are evaluated by a cut off value.
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