BETA 2 MICROGLOBULIN ELISA KIT
INTENDED USE
This Human Beta 2 Microglobulin ELISA Kit is to be used for in vitro quantitative determination of Beta2-Microglobulin (B2MG) concentrations in human serum, plasma and urine samples. The Beta 2 Microglobulin ELISA Kit is intended for RESEARCH USE ONLY and is not for use in diagnostic or therapeutic procedures.
INTRODUCTION
Beta 2 Microglobulin (B2MG) is a small molecule of 11,199dalton (99 amino acids). Its structure and amino acid sequence are identical to the small unit of major histocompatibility complex and similar to the CH3 region of IgG. Elevated serum concentrations suggest increased B2MG production. The phenomenon is seen in patients with autoimmune diseases, viral infections and cancers involving white blood cells, such as lymphoma and multiple myeloma. B2MG content exists in minute amount in urine. Increased B2MG in urine suggests nephrotubulointerstitial disorder or urinary tract infection.
PRINCIPLE OF THE ASSAY
Beta 2 Microglobulin ELISA Kit
Beta 2 Microglobulin ELISA Kit is to be used for in vitro quantitative determination of Beta 2 Microglobulin (B2MG) concentrations in human serum, plasma and urine samples. The Beta 2 Microglobulin ELISA Kit is intended for RESEARCH USE ONLY and is not for use in diagnostic or therapeutic procedures. This Beta 2 Microglobulin ELISA Kit uses a technique called quantitative sandwich immunoassay.
The microtiter plate provided in this Beta 2 Microglobulin ELISA Kit has been pre-coated with a monoclonal antibody specific for B2MG. Standards or samples are added to the microtiter plate wells and B2MG, if present, will bind to the antibody pre-coated on the wells. A preparation of horseradish peroxidase (HRP)-conjugated B2MG antibody is added to each well. The conjugated antibody will bind to the B2MG immobilized on the plate after incubation. All unbound components will be removed by subsequent washing procedure. Next, 3, 3', 5, 5' tetramethyl-benzidine(TMB), a substrate for HRP is added to each well. The colorimetric reaction is proportional to the B2MG content in each well. The reaction is terminated by the addition of a sulphuric acid solution and the colour change is measured pectrophotometrically at the wavelength of 450nm ± 2nm.
In order to quantify the amount of B2MG present in the sample, this Human Beta 2 Microglobulin ELISA Kit includes a set of calibration standards (6 standards). The calibration standards should be assayed at the same time as the samples to allow the operator to produce a standard curve of Optical Density (O.D.) versus B2MG concentration (μg/mL). The concentration of B2MG in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Download Product Data Sheet