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Alanine Aminotransferase Reagent Kit (Colorimetric, Endpoint) Maximize

Alanine Aminotransferase Reagent Kit (Colorimetric, Endpoint)

Alanine Aminotransferase (ALT, SGPT) reagent kit (Colorimetric, Endpoint)(BQ004A-CR) is an assay that is designed for quantitative analysis of human alanine aminotransferase (SGPT, ALT, Glutamate pyruvate transaminase) in serum samples. 

More details

BQ004A-CR

120 tests

£ 250.00

ALT SGPT Reagent Kit (Endpoint, Colorimetric)

Assay Range: 0-120 U/L.
Sensitivity: 0.5 U/L.
Species: Human.
Method: Colorimetric, Endpoint.
Test Size: 120 tests.
Sample Type: Serum.

Principle:
The below enzymatic reaction sequence is employed by this SGPT ALT reagent assay kit:

 

Reagents Supplied: (120 tests)
(A). ALT Colour Developer: 0.5N sodium hydroxide. CORROSIVE!
(B). ALT SUBSTRATE: 0.2 M L-alanine, 2.0 mM a-ketoglutarate, 100 mM phosphate buffer at pH 7.4 + 0.05, 0.2% v/v preservatives.
(C). ALT CALIBRATOR: Solution of sodium pyruvate in 100 mM phosphate buffer at pH 7.4. The activity will be provided in each lot.
(D). ALT COLOUR REAGENT: l.0mM 2,4 dinitrophenylhydrazine in 1N Hydrochloric Acid, preservative.CAUSES BURNS!

Performance Characteristics for this SGPT ALT reagent assay kit:
1. Linearity: 120 IU/L.
2. Sensitivity: Based on an instrument resolution of 0.001absorbance, the present procedure has a sensitivity of 0. 5 IU/L.
3. Comparison: A comparison studies between the present method with a similar method yield a correlation coefficient of 0.97 and a regression equation of y = 0.99 x + 0.71.

Expected Values:
5 - 35 IU/L.

Interfering Substances when this SGPT ALT reagent assay kit:
 - Pyridoxal phosphate can elevate SGPT (ALT) values.
- High levels of serum pyruvate may also interfere with assay performance.
- Young et al. give a list of drugs and other substances that interfere with the determination of SGPT (ALT) activity.

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