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Alanine Aminotransferase Reagent (UV Kinetic) Maximize

Alanine Aminotransferase Reagent (UV Kinetic)

Alanine Aminotransferase (ALT, SGPT) reagent (UV Kinetic)(BQ004B-CR) assay to be used for quantitative detection of human alanine aminotransferase (SGPT, ALT) within serum samples.

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BQ004B-CR

240 tests

£ 300.00

ALT SGPT Reagent (UV-Kinetic)

Assay Range: 0-500 IU/L.
Species: Human.
Method: UV Kinetic.
Test Size: 240 tests (20 x 12ml).
Sample Type: Serum.

Principle:
The below enzymatic reaction sequence is employed by this SGPT, ALT reagent assay:



Reagents Supplied: 240 tests (20 x 12ml).
The reagent provided for the SGPT, ALT reagent contains the following: (Concentrations refer to reconstituted reagent):
- L-Alanine 400 Mm.
- a-Ketoglutarate 13mM.
- LDH 800 U/L.
- NADH 0.2 mM.
- Buffer l00mM at pH 7.5
- Preservatives.
- Non-reactive stabilizers.

Performance Characteristics using this SGPT, ALT reagent assay reagent assay:
1. Linearity: 500 IU/L
2. Comparison: Studies between the present method and a similar method yield a correlation of 0.99 and a regression equation Y = 0.98X + 1.32.

Expected Values:
- Up to 26 IU/L (30 °C).
- Up to 38 IU/L (37 °C).

Interfering Substances when Alanine Aminotransferase reagent assay is used:
 - Pyridoxal phosphate can elevate ALT (SGPT) values.
- High levels of serum pyruvate may also interfere with assay performance.
- Young et al. give a list of drugs and other substances that interfere with the determination of ALT (SGPT) activity.

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