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Aspartate Aminotransferase Reagent Kit (Colorimetric, Endpoint) Maximize

Aspartate Aminotransferase Reagent Kit (Colorimetric, Endpoint)

Aspartate Aminotransferase (AST, SGOT) reagent assay kit (Endpoint, Colorimetric)(BQ006A-CR) a procedure intended to determine quantitatively levels of human AST (SGOT) in serum samples.

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BQ006A-CR

120 tests

£ 250.00

AST (SGOT) Reagent Kit (Colorimetric, Endpoint)

Assay Range: 0-500 IU/L.
Sensitivity: 0.5 IU/L.
Species: Human
Method: Colorimetric, Endpoint
Test Size: 120 tests
Sample Type: Serum

Principle:
The below enzymatic sequence is employed in this AST (SGOT) reagent assay kit:



Reagents Supplied: 120 tests
1. AST Colour Reagent: 0.25% w/v Diazonium salt preserved with formalin.
2. AST Calibrator: A lyophilized serum with SGOT (AST) value provided in each lot.
3. AST Substrate: 33 mM Aspartic acid, 5 mM ketoglutaric acid, phosphate buffer pH 7.4.

Performance Characteristics:
1. Linearity: 500 IU/L.
2. Sensitivity: Based on an instrument resolution of 0.001 absorbance, AST reagent kit procedure has a sensitivity of 0.5 IU/L.
3. Comparison: A comparison study between the present method with an available commercial product using the same identical method on twenty fresh serum samples and two commercial serum controls, ranging from 12 IU/L to 198 IU/L yielded a coefficient of 0.99 and a regression equation of y = l.02 x = 1.89.

Expected Values:
A range of 10 – 40 IU/L at 37 °C was determined using this AST reagent kit.

Interfering Substances:
- Pyridoxal phosphate can elevate AST (SGOT) reagent assay kit values.
- High levels of serum pyruvate may also interfere with the present AST reagent kit performance.

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