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Aspartate Aminotransferase Reagent (UV Kinetic) Maximize

Aspartate Aminotransferase Reagent (UV Kinetic)

Aspartate Aminotransferase (AST, SGOT) reagent assay (UV Kinetic)(BQ006B-CR) a method for quantitatively detecting human AST (SGOT) levels in serum.

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BQ006B-CR

240 tests

£ 300.00

AST (SGOT) Reagent (UV Kinetic)

Assay Range: 0-500 IU/L.
Sensitivity: 0.5 IU/L.
Species: Human
Method: UV Kinetic
Test Size: 240 tests (20 x 12ml)
Sample Type: Serum

Principle:
The below enzymatic sequence is employed in this AST (SGOT) reagent assay:



Reagents Supplied:  240 tests (20 x 12ml)
When reconstituted as directed, this AST reagent contains the following:
- NADH 0.19mM.
- 2-Oxoglutarate 12mM.
- L-Aspartic Acid 200mM.
- MDH 600 U/L.
- LDH 800 U/L.
- Non-reactive preservatives and fillers.
- Buffer 100 mM, pH 7.8 + 0.1.

Performance Characteristics:
1. Linearity: 500 IU/L.
2. Sensitivity: Based on an instrument resolution of 0.001 absorbance, AST reagent assay procedure has a sensitivity of 0.5 IU/L.
3. Comparison: A comparison study between the present AST reagent method with available commercial product using the same method on 22 fresh serum samples from 12 IU/L to 84 IU/L yielded a coefficient of 0.98 and a regression equation of y = 1.04 x - 1.25.

Expected Values:
Up to 28 IU/L (30 °C)
Up to 40 IU/L (37 °C)

Interfering Substances:
- Pyridoxal phosphate can elevate AST (SGOT) reagent assay values.
- High levels of serum pyruvate may also interfere with performance of Aspartate Aminotransferase reagent.

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