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Adrenomedullin (1-50) EIA Kit (Rat) Maximize

Adrenomedullin (1-50) EIA Kit (Rat)

Adrenomedullin (1-50) EIA Kit (Rat)(AM/ADM)(EK-010-08) is a method for quantitatively determining amounts of rat adrenomedullin.

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96 wells

£ 430.00

Rat Adrenomedullin EIA Kit

Specificity : Rat
Sensitivity : 0.1 ng/ml
Linear Range: 0.1-1.79 ng/ml
Range: 0.1-100ng/ml
Size: 96 tests

Reagents Supplied:
- Primary antibody (rabbit anti-peptide IgG)(1 vial).
- 2N HCl (15ml).
- Standard peptide (1 vial).
- Streptavidin-horseradish peroxidase (SA-HRP)(30μl).
- Substrate solution (TMB) (12ml).
- 96 well immunoplate (1 plate).
- 20x assay buffer concentrate (50ml).
- Positive control (2 vials).
- Acetate plate sealer (APS), (3 pieces).
- Biotinylated peptide (1 vial).
- Assay diagram (1 sheet).
- General protocol (1 book).

Intended Use:
Rat Adrenomedullin EIA kit (AM/ADM) is a procedure for quantitative analysis of Adrenomedullin in rat tissue homogenate, serum, plasma, culture media and any other any biological fluid.

Cross Reactivity:
Adrenomedullin (Rat, Human) 100%.
Adrenomedullin (1-50) (Mouse) 100%.
Pro-ADM N20 0%.
Amylin 0%.
Endothelin-1 (ET-1) 0%.

Adrenomedullin (1-50) peptide is associated with pheochromocytoma and was discovered in 1993. AM gene is localized to a single locus on Chromosome 11 with 4 exons and 3 introns. It also shares moderate structural similarity to the calcitonin family of regulatory peptides (calcitonin, CGRP and amylin).

1. Pediatr Res. (2005) 57 (1): 49-55. Adrenomedullin is highly expressed in blood monocytes associated with acute Kawasaki disease: a microarray gene expression study. Nomura, et al.
2. Reprod Biol Endocrinol. (2011) 9: 111. Adrenomedullin in rat follicles and corpora lutea: expression, functions and interaction with endothelin-1. Lei Li, et al.
3. J Immunol. (2006) 176 (9): 5548-58. A Critical Role for Adrenomedullin-Calcitonin Receptor-Like Receptor in Regulating Rheumatoid Fibroblast-Like Synoviocyte Apoptosis. Uzan, et al.
4. Reproductive Biology and Endocrinology (2010) 8 :130. Coexpression of adrenomedullin and its receptor component proteins in the reproductive system of the rat during gestation. Lei Li, et al.
5. J Matern Fetal Neonatal Med. (2013):1-18. Relationship between neonatal adrenomedullin and bilirubin levels. Erdinc K, et al.
6. Eur J Obstet Gynecol Reprod Biol. (2005) 123 (1): 35-40. Vascular expression of adrenomedullin is increased in Wistar rats during early pregnancy. van der Heijden et al.
7. World J Gastroenterol. (2003) 9 (10): 2325-7. Adrenomedullin in cirrhotic and non-cirrhotic portal hypertension. Tahan, et al.
8. Am J Physiol Heart Circ Physiol. (2004) 286 (6) : 2159-68. Expressions of adrenomedullin mRNA and protein in rats with hypobaric hypoxia-induced pulmonary hypertension. Nakanishi, et al.
9. J Sleep Res. (2006) 15 (1): 89-95. Circulating adrenomedullin in obstructive sleep apnoea. Schulz, et al.

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