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Amylin (1-13) EIA Kit Maximize

Amylin (1-13) EIA Kit

Human Amylin (1-13) EIA Kit (Diabetes Associated Peptide [DAP])(EK-017-06) is intended to measure in-vitro quantitatively levels of human amylin.

More details

EK-017-06

96 wells

£ 430.00

Human Amylin EIA Kit (1-13)

Specificity : Human
Sensitivity : 0.12ng/ml
Linear Range: 0.12-1.28ng/ml
Range: 0-100 ng/ml
Size: 96 tests

Reagents Supplied:
- Primary antibody (rabbit anti-peptide IgG)(1 vial).
- 2N HCl (15ml).
- Standard peptide (1 vial).
- Streptavidin-horseradish peroxidase (SA-HRP)(30μl).
- Substrate solution (TMB) (12ml).
- 96 well immunoplate (1 plate).
- 20x assay buffer concentrate (50ml).
- Amylin (1-13) Positive control (2 vials).
- Acetate plate sealer (APS), (3 pieces).
- Biotinylated peptide (1 vial).
- Assay diagram (1 sheet).
- General protocol (1 book).

Intended Use:
Human amylin (1-13) EIA (Diabetes Associated Peptide [DAP]) can be used for amylin measurement in serum, tissue homogenate, plasma, any biological fluid, CSF and urine samples.

Cross Reactivity:
Amylin (1-13) (Human) 100%.
Amylin (Rat, Mouse) 3.5%.
Amylin free acid (Human) 17.9%.
Amylin-Amide (Human) 2.5%.
VIP 0%.
Calcitonin 0%.
Pancreatic Polypeptide 0%.
Insulin 0%.
Pancreastatin (Porcine) 0%.
CGRP 0%.
NPY (Porcine) 0%.
Somatostatin-28 0%.
Glucagon 0%.
Pro-Insulin 0%.

Background:
Amylin has a  vital function along side the endocrine pancreas which contributes to glycemic control. It is a 37-residue peptide hormone which is known to be secreted by pancreatic β-cells at the same time as insulin. Has many functions which include; slowing gastric emptying, promoting satiety, inhibiting the secretion of glucagon during hyperglycemia, thus leading to reduction of the total insulin demand.

References
1. Proc. Natl. Acad. Sci. U.S.A.. (1987) 84: 3881–3885. Amyloid fibrils in human insulinoma and islets of Langerhans of the diabetic cat are derived from a neuropeptide-like protein also present in normal islet cells. Westermark, P., et al.
2. Diabetes. (1991) 40: 1005–1009. Release of amylin from perfused rat pancreas in response to glucose, arginine, β-hydroxybutyrate, and gliclazide. Inoue K., et al.
3. Proc. Natl. Acad. Sci. USA. (1989) 86: 3127–3130. Rat amylin: cloning and tissue-specific expression in pancreatic islets. Leffert J.D., et al.
4. Nature. (1988) 335: 632–635. Pancreatic amylin and calcitonin gene-related peptide cause resistance to insulin in skeletal muscle in vitro. Leighton B. and Cooper G.J.S.
5. Diabetologia. (1978) 15 :417–421. The influence of amyloid deposits on the islet volume in maturity onset diabetes mellitus. Westermark P. and Wilander E.
6. J. Biol. Chem. (1988) 263: 17243–17246. An islet amyloid peptide is derived from an 89-amino acid precursor by proteolytic processing. Sanke T., et al.
7. Diabetes. (1990) 39: 260–265. Induction of insulin resistance in vivo by amylin and calcitonin gene-related peptide. Molina J.M., et al.

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