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BNP-26 EIA Kit Maximize

BNP-26 EIA Kit

BNP-26 EIA Kit (Porcine, Ovine)(EK-011-08) is an in-vitro quantitative protocol designed to determine concentrations of ovine and porcine  Brain Natriuretic Peptide-26 (BNP-26) present.

More details

EK-011-08

96 wells

£ 430.00

BNP-26 EIA Kit (Porcine, Ovine)

Specificity : Porcine, Ovine
Sensitivity : 0.27 ng/ml
Linear Range: 0.27-3.44 ng/ml
Range: 0-100 ng/ml
Size: 96 tests

Reagents Supplied:
- General protocol (1 book).
- 96 well immunoplate (1 plate).
- BNP 26 Standard peptide (1 vial).
- Acetate plate sealer (APS), (3 pieces).
- Biotinylated peptide (1 vial).
- BNP 26 Primary antibody (rabbit anti-peptide IgG)(1 vial).
- Streptavidin-horseradish peroxidase (SA-HRP)(30μl).
- BNP 26 Positive control (2 vials).
- 20x assay buffer concentrate (50ml).
- 2N HCl (15ml).
- Substrate solution (TMB) (12ml).
- Assay diagram (1 sheet).

Intended Use:
BNP-26 EIA Kit (Porcine or Ovine)
is designed to use for measuring quantitatively the levels of Brain Natriuretic Peptide-26 (BNP-26)(Ovine and Porice) in CSF, plasma, tissue homogenate, culture media, any biological fluid, serum or urine.

Assay Principle:
1. Pre-coated wells with secondary antibody and also the non-specific binding sites have been blocked.
2. Secondary antibody is able to bind with the Fc fragment of the primary antibody, the Fab segment is competitively bound by both biotinylated peptides and the peptide standard or the targeted peptide in samples.
3. Biotinylated peptide can then interact with the SA-HRP, which then catalyzes the TMB substrate solution and hydrogen peroxide to form a blue coloured solution.
4. Reaction is stopped by adding hydrogen chloride, which forms a yellow coloured solution.
5. Intensity of the yellow solution is inversely proportional to the amount of the peptide in standard solutions or samples.
6. Unknown concentration of BNP-26 in samples can then be determined using this BNP-26 EIA assay by extrapolation from a standard curve.

Cross Reactivity:
BNP-26 (Porcine or Ovine) 100%.
BNP-32 (Porcine)  100%.
BNP-32 (Rat)  0%.
BNP-32 (Human)  0%.
Alpha ANP (1-28)  0%.
BNP-45 (Rat)  0

Background:
Brain natriuretic peptide (BNP-26) a 26 a.a. polypeptide has been found to be secreted by the ventricles of heart when there is excessive stretching of myocytes. It is release alongside an NT-proBNP (76 amino acids amino terminal fragment). Was first discovered in extracts of porcine brain, whereas within humans it is found to be made in the cardiac ventricles. Elevated levels of BNP 26 and/or NT-proBNP within the blood are useful markers in the diagnosis of heart failure and acute congestive heart failure (CHF).

References
1. Circulation. (2007) 115 (1): 67-75. Hemodynamic Modulation of Endocardial Thromboresistance. Kapur et al.
2. Clin Chem. (2011) 57 (6): 864-73. Secretion of glycosylated pro-B-type natriuretic peptide from normal cardiomyocytes. Tonne JM et al.
3. Vet J. (2008) 178 (1): 85-90. Analytical validation of commercial immunoassays for the measurement of cardiovascular peptides in the dog. Schellenberg S, et al.
4. J Vet Intern Med. (2008) 22 (5): 1118-23. Evaluation of NT-pro BNP and CT-ANP as markers of concentric hypertrophy in dogs with a model of compensated aortic stenosis. Hori Y, et al.
5. Vet J. (2008) 178 (1): 85-90. Analytical validation of commercial immunoassays for the measurement of cardiovascular peptides in the dog. Schellenberg S, et al.
6. J Cardiovasc Pharmacol. (2008) 52 (3): 253-61. Effect of L-2286, a poly(ADP-ribose)polymerase inhibitor and enalapril on myocardial remodeling and heart failure. Bartha et al.

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