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BDNF ELISA Kit Maximize

BDNF ELISA Kit

Human BDNF ELISA Kit (EK-033-22) is an in-vitro method for quantitatively measuring levels of human brain derived neurotrophic factor (BDNF).

More details

EK-033-22

96 wells

£ 430.00

Human BDNF ELISA Kit

Specificity : Human
Sensitivity : 7.8pg/ml
Linear Range: 7.8-500 pg/ml
Range: 0-500 pg/ml
Size: 96 tests

Reagents Supplied:
- General protocol (1 book).
- 96 well immunoplate (1 plate).
- BDNF Standard peptide (1 vial).
- Acetate plate sealer (APS), (3 pieces).
- Biotinylated peptide (1 vial).
- BDNF Primary antibody (rabbit anti-peptide IgG)(1 vial).
- Streptavidin-horseradish peroxidase (SA-HRP)(30μl).
- BDNF Positive control (2 vials).
- 20x assay buffer concentrate (50ml).
- 2N HCl (15ml).
- Substrate solution (TMB) (12ml).
- Assay diagram (1 sheet).

Intended Use:
Human BDNF ELISA Kit
is to be used for quantitative in vitro measurement of human brain derived neurotrophic factor (BDNF) in CSF, plasma, culture media, any biological fluid, serum, tissue homogenate and urine samples.

Cross Reactivity:
BDNF  - 100%.
NGF  - 3%.
NT-3  - 3%.
NT-4/5  - 3%.

Background:
Brain natriuretic peptide was initially identified in porcine brain, but in humans it is found to be made in the cardiac ventricles. It is a 32 amino acid polypeptide secreted by the ventricles of the heart. Biological half-life of BNP is known to be twice as long as that of ANP. Plasma concentrations of BNP is increased in patients with symptomatic and asymptomatic left ventricular dysfunction.

References
1. Therapeutic Advances in Psychopharmacology. (2012) 2 (2): 65-74. Serum brain-derived neurotrophic factor, vascular endothelial growth factor and leptin levels in patients with a diagnosis of severe major depressive disorder with melancholic features. Kotan Z, et al.
2. Cytokine. (2013) 62 (2): 253-61. Evidence of neurotrophic events due to peritoneal endometriotic lesions. Barcena de arellano ML, et al.

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