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BNP-32 EIA Kit Maximize

BNP-32 EIA Kit

Canine BNP-32 EIA Kit (EK-011-22) a procedure for in-vitro quantitatively measuring concentrations of canine brain natriuretic peptice-32 (BNP 32).

More details

EK-011-22

96 wells

£ 430.00

Canine BNP-32 EIA Kit

Specificity : Canine
Sensitivity : 0.44ng/ml
Linear Range: 0.44-8.9 ng/ml
Range: 0-100 ng/ml
Size: 96 tests

Reagents Supplied:
- General protocol (1 book).
- BNP 32 Standard peptide (1 vial).
- Canine BNP-32 Positive control (2 vials).
- Primary antibody (rabbit anti-peptide IgG)(1 vial).
- Acetate plate sealer (APS), (3 pieces).
- 96 well immunoplate (1 plate).
- Streptavidin-horseradish peroxidase (SA-HRP)(30μl).
- Biotinylated peptide (1 vial).
- Substrate solution (TMB) (12ml).
- 20x assay buffer concentrate (50ml).
- 2N HCl (15ml).
- Assay diagram (1 sheet).

Intended Use:
Canine BNP-32 EIA Kit is to be
utilised for in vitro quantitative determination of brain natriuretic peptice-32 (BNP-32) levels in urine, serum, plasma, CSF, any biological fluid, tissue homogenate or culture media.

Assay Principle:
1. Pre-coated wells with secondary antibody and also the non-specific binding sites have been blocked.
2. Secondary antibody is able to bind with the Fc fragment of the primary antibody, the Fab segment is competitively bound by both biotinylated peptides and the peptide standard or the targeted peptide in samples.
3. Biotinylated peptide can then interact with the SA-HRP, which then catalyzes the TMB substrate solution and hydrogen peroxide to form a blue coloured solution.
4. Reaction is stopped by adding hydrogen chloride, which forms a yellow coloured solution.
5. Intensity of the yellow solution is inversely proportional to the amount of the peptide in standard solutions or samples.
6. Unknown concentration of BNP-32 in samples can then be determined using this canine BNP-32 EIA assay by extrapolation from a standard curve.

Cross Reactivity:
BNP-32 (Canine)  100%.
BNP-32 (Porcine)  80%.
BNP-32 (Human or Rat)  0%.
Alpha ANP (1-28)  0%.
Endothelin-1  0%.
CNP-22  0%.

Background:
Brain natriuretic peptide (BNP 32) was first discovered in extracts of porcine brain, whereas within humans it is found to be made in the cardiac ventricles. Is a 32 a.a. polypeptide has been found to be secreted by the ventricles of heart when there is excessive stretching of myocytes. It is release alongside an NT-proBNP (76 amino acids amino terminal fragment). Elevated levels of BNP 32 and/or NT-proBNP within the blood are useful markers in the diagnosis of heart failure and acute congestive heart failure (CHF).

References
1. Vet J. (2008) 178 (1): 85-90. Analytical validation of commercial immunoassays for the measurement of cardiovascular peptides in the dog. Schellenberg S, et al.
2. Clin Chem. (2011) 57 (6): 864-73. Secretion of glycosylated pro-B-type natriuretic peptide from normal cardiomyocytes. Tonne JM et al.
3. J Vet Intern Med. (2008) 22 (5): 1118-23. Evaluation of NT-pro BNP and CT-ANP as markers of concentric hypertrophy in dogs with a model of compensated aortic stenosis. Hori Y, et al.
4. J Cardiovasc Pharmacol. (2008) 52 (3): 253-61. Effect of L-2286, a poly(ADP-ribose)polymerase inhibitor and enalapril on myocardial remodeling and heart failure. Bartha et al.
5. Circulation. (2007) 115 (1): 67-75. Hemodynamic Modulation of Endocardial Thromboresistance. Kapur et al.

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