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ACTH EIA Kit (Human) Maximize

ACTH EIA Kit (Human)

ACTH EIA Kit (Human)(EK-001-01) is an in vitro procedure designed to measure quantitatively the amounts of human adrenocorticotropic hormone (ACTH) present.

More details

EK-001-01

96 wells

£ 430.00

ACTH EIA Kit (Human)

Specificity : Human
Sensitivity : 0.12 ng/ml
Linear Range: 0.12-1.78 ng/ml
Range: 0-25 ng/ml
Size: 96 tests

Reagents Supplied:
- Primary antibody (rabbit anti-peptide IgG)(1 vial).
- 2N HCl (15ml).
- Standard peptide (1 vial).
- Streptavidin-horseradish peroxidase (SA-HRP)(30μl).
- Substrate solution (TMB) (12ml).
- 96 well immunoplate (1 plate).
- 20x assay buffer concentrate (50ml).
- Positive control (2 vials).
- Acetate plate sealer (APS), (3 pieces).
- Biotinylated peptide (1 vial).
- Assay diagram (1 sheet).
- General protocol (1 book).

Intended Use:
Human ACTH EIA Kit is designed to be used for in vitro quantitative measurement of human adrenocorticotropic hormone (ACTH) levels in plasma, serum, culture media, tissue homogenate, CSF, urine or any biological fluid samples.

Cross Reactivity:
ACTH (Human) 100%.
ACTH (Rat) 100%.
ACTH (1-24) (Human) 73%.
ACTH (7-38) (Human) 31%.
β-Endorphin (Human) 1%.
CRF (Human, Rat) 0%,
α-MSH 0%.
Met-Enkephalin 0%.
α-ANP (1-28) (Human, Canine) 0%.
BNP-32 (Human) 0%.

Background:
Adrenocorticotropic hormone (ACTH or corticotropin) is made up of 39 amino acids, the first 13 of which may be cleaved to form α-melanocyte-stimulating hormone (α-MSH). ACTH is a polypeptide hormone produced and secreted by the pituitary gland in response to the release of another hormone, called corticotropin-releasing hormone (CRH), by the hypothalamus. It is found to act through the stimulation of cell surface ACTH receptors, which are primarily located on the adrenocortical cells. It is secreted in several intermittent pulses during the day into the bloodstream and transported around the body. ACTH is released in bursts, so its levels in the blood can vary from minute to minute.  When cortisol levels fall, ACTH levels normally rise. When cortisol levels rise, ACTH levels normally fall. It also increases production of the chemical compounds that trigger an increase in other hormones such as adrenaline and noradrenaline. Adrenocorticotropic is an important player in the hypothalamic-pituitary-adrenal axis.

References
1. Br J Dermatol. (2006) 155 (5): 910-5. Investigation of the corticotropin-releasing hormone-proopiomelanocortin axis in various skin tumours. Kim et al.
2. Traffic. (2007) 8 (7): 867-82. Rab18 Inhibits Secretory Activity in Neuroendocrine Cells by Interacting with Secretory Granules. Vazquez-Martinez et al.
3. Ann N Y Acad Sci. (1999) 885: 268-76. The Role of Proopiomelanocortin-Derived Peptides in Skin Fibroblast and Mast Cell Functions. Teofoli et al.
4. J Gastroenterol. (2007) 42 (7): 522-7. Stress maladjustment in the pathoetiology of ulcerative colitis. Kuroki et al.

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