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Salivary DHEA ELISA Kit Maximize

Salivary DHEA ELISA Kit

Human Salivary DHEA ELISA Kit (DESLV3012) can be used to detect quantitatively amounts of the dehydroepiandrosterone (DHEA) in saliva samples.

More details

DESLV3012

96 wells

£ 280.00

Human Salivary DHEA ELISA Kit

Specificity : Human, Salivary
Sensitivity : 2.186 pg/mL
Assay Range : 0-1440 pg/mL
Size: 96 tests

Reagents Supplied:
- DHEA Microtiter Plate [96 Wells]
- Saliva DHEA Calibrators [6x 1ml vials]
- Saliva DHEA Controls [2x 1ml vials]
- Enzyme Conjugate [1x 14ml]
- Substrate Solution [1x 14ml]
- Stop Solution  [1x 14ml]
- Wash Solution  40x [1x 30ml]

Procedure:
[1]. Prepare the number of microplate wells required to accommodate, controls, calibrators and patient samples.
[2]. Dispense 50μl of each control, standard and sample using new disposable tips for each of the wells.
[3]. Dispense 100μl of enzyme conjugate into each well.
[4]. Mix thoroughly for 10 seconds, it is important to have this step.
[5]. Incubate for 60 minutes at room temperature, shake briskly.
[6]. Rinse the wells 5x with diluted wash solution (400μl per well). Remove as much wash solution as possible by striking the microplate on absorbent paper.  Note: The sensitivity and precision of this assay is markedly influenced by the correct performance of the washing procedure!
[7]. Add 100μl of substrate solution to each of the wells.
[8]. Incubate at room temperature for 20 minutes.
[9]. Stop the reaction by adding 100μl of stop solution to each of the wells.
[10]. Determine the absorbance of each well at 450 nm. It is recommended to read the wells within 10 minutes of completion. Also, it is Important that for each new run a standard curve must be included.

Intended Use:
Human Salivary DHEA ELISA Kit
can be used for detecting in vitro  quantitatively the levels of Dehydroepiandrosterone (DHEA) using saliva as a sample.

Cross-reactivity:
The following materials have been evaluated for cross reactivity.
- DHEA  [100%]
- 17-OH Pregnenolone  [0.072%]
- Progesterone  [0.23%]
- Desoxycorticosterone  [0.052%]
- Androsterone  [0.056%]
- Pregnenolone  [0.013%]
- 11-Desoxycortisol  [0.012%]
- 5-a Dihydrotestosterone  [0.0007%]
- Corticosterone  [0.004%]
- DHEA-S  [0.0037%]
- Aldosterone  [0.0003%]
- Testosterone  [0.002%]
- Cortisol  [0.0007%]
- 17a-Hydroxyprogesterone  [0.0004%]
- Estrone  [non detectable]
- Estradiol 17ß  [non detectable]
- Estriol  [non detectable]
- Estradiol 17a  [non detectable]

Background:
Dehydroepiandrosterone (DHEA) is a steroid which is produced and released mainly through the adrenal cortex and from the gonads to a lesser extent. It is found to function as a precursor in estrogen and testosterone synthesis. The androgenic activity detected is relatively weak (approx 10% of that seen with testosterone). DHEA has low affinity for sex-hormone binding globulin and this property may enhance the physiologic biopotency for DHEA. So far, little in known about the physiologic role of DHEA apart from its enzyme-mediated cell metabolism effects and its anti proliferative effect on many types cell lines. Some studies have suggested that it can affect lipid and cholesterol metabolism, immune function and insulin secretion. Also, abnormal DHEA concentrations have be reported in cases of obesity and schizophrenia.

References
1. Horm Behav, (2005) 48 (2) 187-195. Azurmendi A, et al.
2. Complement Ther Med, (2006) 14 (2) 127-132. Laidlaw TM, et al.
3. Horm Behav, (2006) 50 (1) 132-140. Azurmendi A, et al.
4. J Psychopharmacol, (2006) 20 (5) 643-649. Gallagher P., et al.
5. Psychopharmacology (Berl), (2006) 188 (4) 541-551. Alhaj HA., et al.
6. J Headache Pain, (2006) 7 (2) 90-94. Patacchioli FR, et al.
7. Horm Behav, (2006) 49 (4) 478-483. Whembolua GL, et al.
8. Am J Hum Biol, (2006) 18 (5) 639-653. Bond LJ, et al.

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