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Androstenedione Free In Saliva ELISA Kit Maximize

Androstenedione Free In Saliva ELISA Kit

Androstenedione Free Saliva ELISA Kit (DESLV4780) an in vitro colorimetric procedure to quantitatively analyse amounts of androstenedione free present using saliva samples.

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DESLV4780

96 wells

£ 280.00

Androstenedione Free Saliva ELISA Kit

Specificity : Human, Saliva
Sensitivity : 5 pg/mL
Assay Range : 0-1000 pg/ml
Size: 96 tests

Reagents Supplied:
- Androstenedione Coated Microtiter Plate [96 Wells]
- Androstenedione Standards [5x 1ml vials]
- TMB Substrate Solution [1x 12ml]
- Enzyme Conjugate [1x 0.4ml]
- Stop Solution  [1x 12ml]
- Incubation Buffer  [1x 30ml]

Procedure:
This androstenedione free saliva ELISA is a competitive solid phase enzyme-linked immunosorbent assay. As it is necessary to perform the determination in duplicate, prepare two wells for each of the five points of the standard curve (S0-S4), two for each sample, one for Blank.
[1]. Add 50μl [Sample or Standard S0-S4] or 0μl for Blank.
[2]. Follow by 150μl dilute conjugate.
[3]. Incubate at +37°C for 1 hour. Remove the contents from each well. Wash the wells with 300 μL of distilled water. Repeat the washing procedure by draining the water completely.
[4]. Add 100μl TMB Substrate, Incubate in the dark at room temperature 22 °C - 28 °C for 15 minutes.
[5]. Stop the reaction by adding 100μl of stop solution.
[6]. Read the absorbance (E) at 450 nm against Blank.

Intended Use:
Androstenedione Free In Saliva ELISA is a colorimetric competitive immunoenzymatic procedure which can be used for quantitative measurement of Androstenedione free levels in saliva.


Cross-reactivity:
The cross reaction of the antibody calculated at 50% according to Abraham is shown below.
- Androstenedione Saliva  [100%]
-Testosterone  [1.2%]
- Epitestosterone  [0.2%]
- 5α-dihydrotestosterone  [0.1%]
- DHEA  [0.1%]
- Progesterone  [1x10 -3 %]
- Estrone  [1x10-3 %]
- Cortisol  [1x10-3 %]

References
1. Fertility and Sterility, (1987) 48(1): 78. Venturoli S., et al.
2. Endocr. Reviews, (1982) 3 (4): 304-367. Riad D., et al.
3. J. Clin. Endoc.& Metab., (1973) 36: 475. Judd H. and Yen S.
4. Hormone Res., (1986) 24: 269. Venturoli S., et al.
5. J. Clin. Endoc. & M. (1974) 39: 340. Abraham G.
6. 79th Year book Medical (1985) Publishers Inc: Chicago. Hillier S.G7. Acta Obstet. Gynecol Scand (1981) 60: 417 -420. Fisher-Rasmussen, W., Gabrielsen, M. V., and Wisborg.
8. Acta Obstet Gynecol Scand (1981) 60: 187. Bagger, P. V., Jacobsson, K., and Gullberg, B.
9. Clin. Chem. (1979) 25 (5): 716. Osterman, T. M., Juntunen, K.O., and Gothoni, G.D.
10. J. Clin. Endoc. Metab. (1983) 56: 454. Vining, R. F., Mc Ginley, R., and Rice B.

Please contact us if you require the complete protocol insert for this Androstenedione Free Saliva ELISA Kit

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