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MCP-3 ELISA Kit Maximize

MCP-3 ELISA Kit

Human MCP-3 ELISA Kit (EL10016) is designed for in-vitro quantitative measurement of monocyte chemotactic protein-3 (MCP-3).

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EL10016

96 wells

£ 390.00

Human Monocyte Chemotactic Protein-3 ELISA Kit [MCP-3]

Specificity : Human
Sensitivity : Less than 30.0pg/mL
Assay Range : 30-1600pg/mL
Size: 96 tests.

Reagents Supplied:
- Human MCP-3 Microtiter Plate  [96 Wells]
- Human MCP-3 Standard  [2 vials]
- Human MCP-3 Conjugate  [15ml]
- Substrate A  [11ml]
- Substrate B  [11ml]
- Calibrator Diluent I  [22ml]
- Calibrator Diluent II  [22ml]
- Wash Buffer 20x [60ml]
- Sample Diluent  [12ml]
- Stop Solution  [14ml]

Intended Use:
Human MCP-3 ELISA Kit is a method for in vitro quantitatively determining monocyte chemotactic protein-3 concentrations in plasma, serum, cell supernatant and any biological fluids.

Sensitivity:
Using calibrator diluent I it's mimimum detection level is 30.0pg/mL and using calibrator diluent II it is 20.0pg/mL.

Specificity:
This human monocyte chemotactic protein-3 ELISA is useful in detecting either recombinant and or natural MCP-3.

Cross-Reactivity:
It exhibits no cross reactivity with any of the following substances (human specific): IL-8, IL-1beta, RANTES, SAA, MCAF, TNF-alpha, TGF-beta, GM-CSF, M-CSF, EGF, FGF or EPO.

International Calibration Standard:
Human ELISA MCP-3 has been calibrated against a highly purified E. Coli expressed as a polypeptide form (76 a.a.) of recombinant human MCP-3.

Expected Normal Values:
Analysis of normal healthy individuals gave the following results:
1. Serum or plasma (n=14): mean was More than 30pg/mL.
2. Urine (n=14): mean was Above 20pg/mL.

Reference
1. Genomics. (1994) 21: 403. Opdenakker, G. et al.
2. J. Biological Chemistry. (1996) 27(18):10521. Jiang-Hong, G.
3. J. of Biol. Chem. (1995) 270 (38): 22123. Ben-Baruch, S. et al.
4. Eur. Cytokine Netw. (1993) 4, 99-110. Minty, A. et al.
5. J. of Interferon and Cytokine Res. (1995) 15:955. Nasur, S. et al.
6. J. Exp. Med. (1994) 179: 751. Dahinden, C.A. et al.
7. Biochem. Biophys. Res. Commun. (1993) 191: 535. Opdenakker, G.,et al. 
8. Biochem. Biophy. Res. Comm. (1994) 201: 493. Thirion, S. et al.
9. Eur. J. Immun. (1995) 25: 2612. Xu, L.L. et al.
10. J. Exp. Med. (1992) 176: 59. Van Damme, J. et al.
11. Immunol. (1994) 152: 3615. Sozzani, S. et al.
12. Advances in Immunology. (1994) 55:97. Baggiolini, M. et al.

Please contact us if you require any further information on this Human Monocyte Chemotactic Protein-3 ELISA Kit [MCP-3]

  • MCP-3 ELISA
    Detailed protocol insert for this ELISA kit for Human Monocyte Chemotactic Protein-3 (MCP-3)
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