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Adrenaline ELISA Kit

Human Adrenaline ELISA Kit (RE59251) an in-vitro method for quantitative diagnostic measurement of human adrenaline (epinephrine) levels.

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RE59251

96 wells

£ 410.00

Human Adrenaline ELISA Kit (Epinephrine)

Specificity : Human
Sensitivity : 1.5 ng/mL
Assay Range : 1.5 - 150 ng/mL
Size: 96 tests

Reagents Supplied:
- Coated Microtiter Plate [96 Wells]
- Adrenaline Standards  [6x 2.5ml]
- Adrenaline Controls  [2x 2.5ml]
- Enzyme Conjugate Conc.  50x  [400μl]
- Extraction Plate  [2x 24]
- Extraction Buffer  [60ml]
- COMT  [2x 1.25ml]
- Coenzyme Solution  [2x 1.25ml]
- Enzyme Buffer  [3ml]
- Release Buffer  [2x 13ml]
- Acylation Reagent  [3ml]
- Wash Buffer Conc. 10x [100ml]
- Epinephrine Antiserum  [8ml]
- COMT Additive [2ml]
- PNPP Substrate Solution  [25ml]
- PNPP Stop Solution  [15ml]
- Adhesive Foil  [3x]

Key Features:
[1]. Method:  ELISA
[2]. Kit Size: 12x8 Break apart strips.
[3]. Standard Range: 1.5 – 150 ng/mL
[4]. Incubation Time: 1 x 2 h, 1 x 1 h, 1 x 40 min
[5]. Substrate: PNPP 405nm
[5]. Volumes/Specimen: 20μl urine and 500μl plasma
[6]. Regulatory Status: CE, EU, IVD, CDN

Intended Use:
Human Adrenaline ELISA kit [Epinephrine] is to be utilised for in vitro diagnostic quantitative detection of human adrenaline (epinephrine) in human plasma or urine. Can use for both manual or automated immunoassay system.

Principle:
Solid phase adrenaline ELISA based on the sandwich principle. The wells are coated with a goat anti rabbit antibody. The added liquid antibody, directed towards an epitope of an antigen molecule binds to the plate within the incubation time. The antigen of the sample is incubated in the coated well with enzyme conjugated second antibody (E-Ab), directed towards a different region of the antigen molecule. After the substrate reaction the intensity of the developed color is proportional to the amount of the antigen. Results of samples can be determined directly using the standard curve.

Expected Values:
Healthy subjects show the following Adrenaline (Epinephrine) values: (5 % - 95 % percentile) using this human adrenaline ELISA:
[1]. Urine:  below 20 μg/d and less than 110 nmol/d.
[2]. Plasma: below 125 pg/mL and less than 0.68 nmol/L
It is recommended that each laboratory establishes its own range of normal values human epinephrine (adrenaline).

Limitations:
The following blood components do not have a significant effect (+/-20% of expected) on the test results up to the below stated concentrations:
- Hemoglobin  [2.0 mg/mL]
- Bilirubin  [1.0 mg/mL]
- Triglyceride  [91 mg/mL]

Cross Reactivity using this human adrenaline ELISA kit:
[1]. Adrenaline [Epinephrine]  [100%]
[2]. Noradrenaline  [0.23%]
[3]. Metanephrine  [0.005%]
[4]. Normetanephrine  [0.001%]
[5]. Other substances tested  [0.06 %]

Background:
The chemical structure of the catecholamines is identical in all animals. Catecholamines adrenalin, noradrenalin and dopamine are all synthesized in the adrenal medulla, in the brain and the sympathetic nervous system. They are known to influence virtually all tissues and are involved together with other hormonal and neuronal systems in the regulation of a wide variety of physiological processes.

As catecholamines and their metabolites metanephrine and normetanephrine are secreted in increasing amounts in a number of diseases, they may be used for diagnostic purposes. In this context, diagnosis and the follow-up of tumor diseases of the nervous system are of special importance. This applies primarily to the pheochromocytoma, but also the neuroblastoma and the ganglioneuroma.

Reference
1. Circulation Research, January (2006). Rust MB, Faulhaber J et. al.
2. Clin. Biochem., (2004) QML Pathology, Brisbane QLD. Creces J.,
3. Clin. Lab., (2002) 48: 61-71. Westermann J, Hubl W, Kaiser N, Salewski L.

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