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Beta-2 Microglobulin ELISA Kit Maximize

Beta-2 Microglobulin ELISA Kit

Human Beta-2 Microglobulin ELISA Kit (EL10051) is a quantitative protocol to investigate the concentrations of beta 2 Microglobulin (B2M) present.

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EL10051

96 wells

£ 395.00

Human B2M ELISA Kit [Beta-2 Microglobulin]

Specificity : Human
Sensitivity : Less than 0.2μg/mL
Assay Range : 0.75-12μg/mL
Size: 96 tests.


Reagents Supplied:
- Human B2M Microtiter Plate  [96 Wells]
- Human B2M Standard  [6 vials]
- Human B2M Conjugate  [12ml]
- Substrate A  [10ml]
- Substrate B  [10ml]
- Sample Diluent [60ml]
- Stop Solution [10ml]

Intended Use:
Human beta-2 Microglobulin ELISA to be used for the in vitro quantitative study of beta 2 Microglobulin (B2M) concentrations in serum, plasma and urine.

Sensitivity:
The calculated minimum level of detection was 0.2μg/mL.

Recovery: This has been estimated to be in the range of 94-105%.

Precision:
1. Run- between (n=16): CV reading of 8.4% (average concentration of 0.536μg/mL).
2. Run- within (n=16): CV value of 7.43% (mean concentration of 0.526μg/mL).

Specificity:
This human B2M ELISA kit does not exhibit any cross reactivity with any other substances using human serum samples: PSA, CEA, human IgG, CRP and human serum albumin.

Hook Effect:
No hook effect was detectable.

International Calibration Standard:
Human ELISA beta-2 Microglobulin has been calibrated against NIBSC/WHO B2MG First International Standard (Case Number. 96/668).

Expected Normal Values:
B2MG normal value were determined using more than 70 plasma and serum which had been taken from normal blood donor patients. The Beta-2 Microglobulin concentration recorded was between the range of 0.267 to 1.278μg/mL. Urine samples were also tested and it was found to contain less than 0.2μg/mL. However, we would recommend that each laboratory determines its own normal range using its own individual population.

Reference
1. Rheumatol Int (1982) 2:145-49. Maury CPJ, Helve T and Sjoblom C.
2.. Biochem (1973)12: 4811-22. Cunningham BA, Wang JL, Berggard I, Peterson PA
3. Acta Med Scand (1986) 219: 489 – 95. Sandberg T, et al.
4. Infection (1988)16:109-10. Morfeldt-Manson J, et al.
5. J Exp Med (1973) 138:1608-12. Grey HM., et al.
6. Ann Rheum Dis (1978) 37: 328-32. Manicourt D, Brauman H, and Orloff S.
7. Cancer (2006) 51:2220-25. Hagberg H, Killander A, Simmonsson B.

Please contact us if you require any further information on this Human B2M ELISA Kit [Beta-2 Microglobulin]

  • Human B2M ELISA
    Detailed protocol insert for this ELISA kit for Human Beta-2 Microglobulin (B2M)
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