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Adiponectin ELISA Kit Maximize

Adiponectin ELISA Kit

Human Adiponectin ELISA Kit (DEE009) is a quantitative in-vitro method designed to analyse levels of human adiponectin present.

More details

DEE009

96 wells

£ 430.00

Human Adiponectin ELISA Kit

Specificity : Human
Sensitivity : 0.6 ng/ml
Assay Range : 2 - 100 ng/ml
Size: 96 tests

Reagents Supplied:
- Microtiter plate  [96 Wells]
- Native Adiponectin Standards A-E [Lyophilised]
- Dilution buffer  [125ml]
- Adiponectin Control Serum  [2x 500μl lyophilised]
- Antibody-POD-Conjugate  [12ml]
- Washing Buffer Conc. 20x  [50ml]
- TMB Substrate Solution  [12ml]
- Stopping Solution  [12ml]

Key Features:
[1]. Method:  ELISA
[2]. Kit Size: 96 Wells.
[3]. Standard Range:  1 – 100 ng/ml
[4]. Incubation Time: 60 min, 30 min, 15 min.
[5]. Substrate: TMB 450nm
[6]. Volume/Specimen: 100μl after dilution serum or plasma
[7]. Sample Preparartion: Dilution 1:500

Intended Use:
Human Adiponectin ELISA is an enzyme immunoassay for quantitative Determination of human adiponectin in human serum and plasma samples.

Specificity:
There was no cross reactivity observed with the following species:
Chicken, Guinea pig, Dog, Horse, Mouse, Rabbit, Donkey, Cow, Cat, Goat, Sheep and Rat.

Interference:
No interference was observed for the below substance below the concentrations shown:
- Bilirubin [100μg/ml]
- Triglcerides [100mg/ml]
- Hemolysat  [100μg/ml]

References
1. Endocrinology (2004) 145 (12): 5589-97. Induction of adiponectin in skeletal muscle by inflammatory cytokines: in vivo and in vitro studies. Delaigle A.M., et al.
2. J Biochem (Tokyo), (1996) 120 (4): 803-12. Isolation and characterization of GBP28, a novel gelatin-binding protein purified from human plasma. Nakano Y., et al.
3. Biochem Biophys Res Commun. (2004) 315 (2): 264-71. Adiponectin suppresses proliferation and superoxide generation and enhances eNOS activity in endothelial cells treated with oxidized LDL. Motoshima H., et al.
4. Clin Endocrinol (Oxf)(2004) 61 (6): 753-9. Correlations of adiponectin level with insulin resistance and atherosclerosis in Japanese male populations. Higashiura K., et al.
5. J Gerontol A Biol Sci Med Sci, (2004) 59 (9): 935-9. Adipocytokines, fat distribution, and insulin resistance in elderly men and women. Zoico E., et al.
6. Diabetes Care (2004) 27 (7): 1721-7. Plasma adiponectin, insulin sensitivity, and subclinical inflammation in women with prior gestational diabetes mellitus. Winzer C., et al.
7. Biochem Biophys Res Commun (2004) 323 (2): 630-5. Adiponectin induces the anti-inflammatory cytokines IL-10 and IL-1RA in human leukocytes. Wolf, A.M., et al.
8. Nat Med, (2002) 8 (11): 1288-95. Adiponectin stimulates glucose utilization and fatty-acid oxidation by activating AMPactivated protein kinase. Yamauchi T., et al.
9. T. Miyazaki, and H. Daida, Clin Chim Acta, (2004) 344 (1-2): 1-12. Adiponectin and atherosclerotic disease. Shimada K., et al.
10. Clin Endocrinol (Oxf)(2004) 61 (6): 738-46. Adiponectin is independently associated with insulin sensitivity in women with polycystic ovary syndrome. Spranger J., et al.

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