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ACTH EIA Kit (Mouse, Rat) Maximize

ACTH EIA Kit (Mouse, Rat)

ACTH EIA Kit (Mouse, Rat)(EK-001-21) is designed to be used to quantitatively measure in vitro concentrations of adrenocorticotropic hormone (ACTH).

More details

EK-001-21

96 wells

£ 430.00

ACTH EIA Kit (Mouse, Rat)

Specificity : Mouse, Rat
Sensitivity : 0.08ng/ml
Linear Range: 0.08-1.78ng/ml
Assay Range : 0-25ng/ml
Size: 96 tests.

Reagents Supplied:
- Primary antibody (rabbit anti-peptide IgG)(1 vial).
- 2N HCl (15ml).
- Standard peptide (1 vial).
- Streptavidin-horseradish peroxidase (SA-HRP)(30μl).
- Substrate solution (TMB) (12ml).
- 96 well immunoplate (1 plate).
- 20x assay buffer concentrate (50ml).
- Positive control (2 vials).
- Acetate plate sealer (APS), (3 pieces).
- Biotinylated peptide (1 vial).
- Assay diagram (1 sheet).
- General protocol (1 book).

Intended Use:
ACTH EIA kit can be utilised for quantitative in vitro analysis of mouse or rat adrenocorticotropic hormone (ACTH) in plasma, culture media, tissue homogenate, CSF, serum, urine and any biological fluid samples.

Cross Reactivity:
ACTH (Rat, Mouse) 100%.
ACTH (Human) 100%.
ACTH (18-39) (Human) 100%.
ACTH (1-24) (Human) 0.01%.
ACTH (7-38) (Human) 0%.
β-Endorphin (Rat) 0%.
α-MSH 0%.
LH-RH 0%.
PACAP-38 (Ovine) 0%.

Background:
Adrenocorticotropic hormone (ACTH or corticotropin) is made up of 39 amino acids, the first 13 of which may be cleaved to form α-melanocyte-stimulating hormone (α-MSH). ACTH is a polypeptide hormone produced and secreted by the pituitary gland in response to the release of another hormone, called corticotropin-releasing hormone (CRH), by the hypothalamus. It is found to act through the stimulation of cell surface ACTH receptors, which are primarily located on the adrenocortical cells. It is secreted in several intermittent pulses during the day into the bloodstream and transported around the body. ACTH is released in bursts, so its levels in the blood can vary from minute to minute.  When cortisol levels fall, ACTH levels normally rise. When cortisol levels rise, ACTH levels normally fall. It also increases production of the chemical compounds that trigger an increase in other hormones such as adrenaline and noradrenaline. Adrenocorticotropic is an important player in the hypothalamic-pituitary-adrenal axis.

References
1.  Inflamm Res. (2004) 53 (7): 269-76. Involvement of arginine vasopressin in endogenous central histamine-induced reversal of critical haemorrhagic hypotension in rats. Jochem.
2. Life Sci. (2007) 80 (8): 725-31. Molecular aspects of adrenal regulation for circadian glucocorticoid synthesis by chronic voluntary exercise. Otawa et al.
3. Dis Model Mech. (2012) 5 (5):681-5. Fasting hyperglycemia in the Goto-Kakizaki rat is dependent on corticosterone: a confounding variable in rodent models of type 2 diabetes. Sara A., et al.
4. Am J Physiol Regul Integr Comp Physiol. (2008) 295 (3): 899-905. Dietary sodium manipulation during critical periods in development sensitize adult offspring to amphetamines. McBride et al.
5. Neuroscience. (2005) 131 (3): 635-45. Alterations in stress-associated behaviors and neurochemical markers in adult rats after neonatal short-lasting local inflammatory insult. Anseloni et al.
6. Brain Behav Immun. (2006) 20 (5): 460-8. Regulation of adrenal glucocorticoid synthesis by interleukin-10: a preponderance of IL-10 receptor in the adrenal zona fasciculata. Koldzic-Zivanovic et al.
7. Life Sci. (2008) 82 (15-16): 862-8. Effect of chronic stress on gastric emptying and plasma ghrelin levels in rats. Ochi et al.
8. Neurosci Lett. (2005) 383 (1-2) :17-21. Up-regulation of the expression of cocaine and amphetamine-regulated transcript peptide by electroacupuncture in the arcuate nucleus of diet-induced obese rats. Tian et al.
9. Regul Pept. (2007) 138 (2-3): 141-4. Neither intravenous nor intracerebroventricular administration of obestatin affects the secretion of GH, PRL, TSH and ACTH in rats. Yamamoto et al.
10. Neuroscience. (2008) 156 (3): 466-74. The single-prolonged stress paradigm alters both the morphology and stress response of magnocellular vasopressin neurons. Yoshii et al.
11. Mol Endocrinol. (2008) 22 (8): 1924-34. Aquaporin 1 is important for maintaining secretory granule biogenesis in endocrine cells. Arnaoutova et al.

  • ACTH EIA Kit
    Detailed protocol insert for this ACTH EIA kit for mouse and rat samples.
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