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Adrenomedullin (1-50) EIA Kit Maximize

Adrenomedullin (1-50) EIA Kit

Human Adrenomedullin (1-50) EIA Kit (EK-010-01) is an in-vitro quantitative method intended to measure the concentrations of Adrenomedullin human levels.

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EK-010-01

96 wells

£ 430.00

Human Adrenomedullin EIA Kit

Specificity : Human
Sensitivity : 0.13 ng/ml
Linear Range: 0.13-1.47 ng/ml
Assay Range : 0-100ng/ml
Size: 96 tests.

Reagents Supplied:
- Primary antibody (rabbit anti-peptide IgG)(1 vial).
- 2N HCl (15ml).
- Standard peptide (1 vial).
- Streptavidin-horseradish peroxidase (SA-HRP)(30μl).
- Substrate solution (TMB) (12ml).
- 96 well immunoplate (1 plate).
- 20x assay buffer concentrate (50ml).
- Positive control (2 vials).
- Acetate plate sealer (APS), (3 pieces).
- Biotinylated peptide (1 vial).
- Assay diagram (1 sheet).
- General protocol (1 book).

Intended Use:
Human Adrenomedullin (1-50) EIA Kit (Cat. No.# EK-010-01) in vitro quantitative determination of Adrenomedullin (1-50) levels in serum, plasma, culture media and various other biological fluids samples.

Assay Principle:
1. Human Adrenomedullin EIA kit has been pre-coated with secondary antibody and also the non-specific binding sites have been blocked.
2. Secondary antibody is able to bind with the Fc fragment of the primary antibody, the Fab segment is competitively bound by both biotinylated peptides and the peptide standard or the targeted peptide in samples.
3. Biotinylated peptide can then interact with the SA-HRP, which then catalyzes the TMB substrate solution and hydrogen peroxide to form a blue coloured solution.
4. Reaction is stopped by adding hydrogen chloride, which forms a yellow coloured solution.
5. Intensity of the yellow solution is inversely proportional to the amount of the peptide in standard solutions or samples.
6. Unknown concentration in samples can then be determined using this adrenomedullin (1-50) EIA assay kit by extrapolation from a standard curve.

Cross Reactivity:
Adrenomedullin (1-52)(Human) 100%.
Adrenomedullin (1-50)(Rat, Mouse) 100%.
Adrenomedullin (13-52)(Human) 100%.
Adrenomedullin-Gly (Human) 1%.
Amylin 0%.
CGRP 0%.
CGRP II 0%
Endothelin-1 0%.
α-ANP (1-28) 0%.
BNP-32 0%.
CNP-22 0%.

Background:
Adrenomedullin gene is localized to a single locus on Chromosome 11 with 4 exons and 3 introns. It is a peptide associated with pheochromocytoma and was discovered in 1993. Mature AM is activated to form a 52 amino acid, 6-amino acid ring, that shares moderate structural similarity to the calcitonin family of regulatory peptides (calcitonin, CGRP and amylin).

References
1. J Sleep Res. (2006) 15 (1): 89-95. Circulating adrenomedullin in obstructive sleep apnoea. Schulz, et al.
2. J Dent. (2007) 35 (8): 636-42. Dissolution of bio-active dentine matrix components by mineral trioxide aggregate. Tomson et al.
3. Clin Diagn Lab Immunol. (2003) 10 (4): 546-51. Adrenomedullin expression by gastric epithelial cells in response to infection. Allaker, et al.
4. J Matern Fetal Neonatal Med. (2013):1-18. Relationship between neonatal adrenomedullin and bilirubin levels. Erdinc K, et al.
5. The Journal of Clinical Endocrinology & Metabolism. (2012): 2011-3290. Possible Role of Adrenomedullin In the Pathogenesis of Tubal Ectopic Pregnancy. Liao SB, et al.
6. Clin Exp Hypertens. (2013):1-5. Anxiety disorders are associated with increased plasma adrenomedullin level and left ventricular hypertrophy in patients with hypertension. Kong DG., et al.
7. Fertil Steril. (2013)100 (2): 459-463. Decreases in adrenomedullin expression and ciliary beat frequency in the nasal epithelium in tubal pregnancy. WS, Li HW, Liao SB, et al.
8. Fertil Steril. (2006) 86 (4): 942-8. Plasma adrenomedullin levels in patients with polycystic ovary syndrome. Ucar, et al.
9. J Endocrinol. (2001) 170 (1): 259-65. Autocrine role of adrenomedullin in the human adrenal cortex. Thomson et al.
10. World J Gastroenterol. (2003) 9 (10): 2325-7. Adrenomedullin in cirrhotic and non-cirrhotic portal hypertension. Tahan, et al.
11. J Invest Dermatol. (2001) 117 (2): 353-9. Regulation of adrenomedullin secretion in cultured human skin and oral keratinocytes. Kapas, et al.
12. Pediatr Res. (2005) 57 (1): 49-55. Adrenomedullin is highly expressed in blood monocytes associated with acute Kawasaki disease: a microarray gene expression study. Nomura, et al.
13. J Dent Res. (2004) 83 (4): 333-7. Expression of adrenomedullin and its receptors in human salivary tissue. Kapas, et al.

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