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AGRP (83-132) NH2 EIA Kit Maximize

AGRP (83-132) NH2 EIA Kit

Human AGRP (83-132) NH2 EIA Kit (EK-003-53) protocol designed for quantitatively measuring human agouti-related protein (AGRP)(83-132) NH2 concentrations.

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EK-003-53

96 wells

£ 560.00

Human AGRP Amide EIA Kit

Specificity : Human
Sensitivity : 0.09 ng/ml
Linear Range: 0.09-0.61ng/ml
Assay Range : 0-100ng/ml
Size: 96 tests.

Reagents Supplied:
- Primary antibody (rabbit anti-peptide IgG)(1 vial).
- 2N HCl (15ml).
- Standard peptide (1 vial).
- Streptavidin-horseradish peroxidase (SA-HRP)(30μl).
- Substrate solution (TMB) (12ml).
- 96 well immunoplate (1 plate).
- 20x assay buffer concentrate (50ml).
- Positive control (2 vials).
- Acetate plate sealer (APS), (3 pieces).
- Biotinylated peptide (1 vial).
- Assay diagram (1 sheet).
- General protocol (1 book).

Intended Use:
Human AGRP NH2 EIA Kit can be used for quantitative analysis of Agouti-Related Protein levels in plasma, serum, any biological fluid samples, CSFand urine.

Assay Principle:
1. Human AGRP amide EIA kit has been pre-coated with secondary antibody and also the non-specific binding sites have been blocked.
2. Secondary antibody is able to bind with the Fc fragment of the primary antibody, the Fab segment is competitively bound by both biotinylated peptides and the peptide standard or the targeted peptide in samples.
3. Biotinylated peptide can then interact with the SA-HRP, which then catalyzes the TMB substrate solution and hydrogen peroxide to form a blue coloured solution.
4. Reaction is stopped by adding hydrogen chloride, which forms a yellow coloured solution.
5. Intensity of the yellow solution is inversely proportional to the amount of the peptide in standard solutions or samples.
6. Unknown concentration in samples can then be determined by extrapolation from a standard curve.

Cross Reactivity:
AGRP (83-132)-NH2 (Human) 100%.
AGRP Form C-NH2 (Human) 100%.
AGRP (82-131)- NH2 (Mouse) 65%.
Leptin (Human) 0%.
Orexin A 0%
Orexin B (Human) 0%.
Neuropeptide Y 0%.
α-MSH 0%.
MCH 0%.
CGRP 0%.

Background:
Agouti-related peptide (AGRP) is a neuropeptide produced in the brain by the AGRP/NPY neuron. AGRP has been shown to be an inverse agonist of melanocortin receptors, specifically, MC3-R and MC4-R. Understanding the function AGRP plays in weight gain could assist in help reduce obesity. It increases appetite and decreases metabolism and energy expenditure.

References
1. J Neurol. (2013) 260 (2): 475-83. Role of intestinal peptides and the autonomic nervous system in postprandial hypotension in patients with multiple system atrophy. Takeshi Fukushima, et al.
2. Peptides. (2007) 28 (5): 1035-9. Plasma agouti-related protein (AGRP), growth hormone, insulin responses to a single circuit-resistance exercise in male college students. Ghanbari-Niaki, et al.
3. Neurosci Lett. (2004) 370 (2-3): 108-13. The effects of agouti-related protein gene transfer in vivo by electroporation in mice. Xiang, et al.

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