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Amylin EIA Kit (Rat, Mouse) Maximize

Amylin EIA Kit (Rat, Mouse)

Amylin EIA Kit (Rat, Mouse)(EK-017-11) is a quantitative procedure for analysing levels of amylin in either mouse or rat samples.

More details

EK-017-11

96 wells

£ 430.00

Amylin EIA Kit

Specificity : Mouse, Rat
Sensitivity : 0.24ng/ml
Linear Range: 0.24-3.11ng/ml
Assay Range : 0-100ng/ml
Size: 96 tests.

Reagents Supplied:
- Primary antibody (rabbit anti-peptide IgG)(1 vial).
- 2N HCl (15ml).
- Standard peptide (1 vial).
- Streptavidin-horseradish peroxidase (SA-HRP)(30μl).
- Substrate solution (TMB) (12ml).
- 96 well immunoplate (1 plate).
- 20x assay buffer concentrate (50ml).
- Positive control (2 vials).
- Acetate plate sealer (APS), (3 pieces).
- Biotinylated peptide (1 vial).
- Assay diagram (1 sheet).
- General protocol (1 book).

Intended Use:
Amylin EIA Kit (Rat and Mouse) is a protocol for in vitro quantitative determination of amylin amounts in plasma, serum, any biological fluid, urine, CSF and tissue homogenate.

Assay Principle:
1. Rat and Mouse Amylin EIA kit has been pre-coated with secondary antibody and also the non-specific binding sites have been blocked.
2. Secondary antibody is able to bind with the Fc fragment of the primary antibody, the Fab segment is competitively bound by both biotinylated peptides and the peptide standard or the targeted peptide in samples.
3. Biotinylated peptide can then interact with the SA-HRP, which then catalyzes the TMB substrate solution and hydrogen peroxide to form a blue coloured solution.
4. Reaction is stopped by adding hydrogen chloride, which forms a yellow coloured solution.
5. Intensity of the yellow solution is inversely proportional to the amount of the peptide in standard solutions or samples.
6. Unknown concentration in samples can then be determined by using this amylin EIA assay to extrapolation from a standard curve.

Cross Reactivity:
Amylin-Amide (Feline) 100%.
Amylin (Rat) 100%.
Amylin Amide (Human) 10%.
Amylin (Human) 0%.
Proinsulin 0%.
CGRP II  0%.
Insulin 0%.
Glucagon  0%.
Pancreatic Polypeptide 0%.
VIP 0%.
Somatostatin 28 0%.
NPY (Porcine) 0%.
CGRP  0%.

Background:
Amylin has a vital function along side the endocrine pancreas which contributes to glycemic control. It's a 37-residue peptide hormone which is known to be secreted by pancreatic β-cells at the same time as insulin. It has many functions which include; slowing gastric emptying, promoting satiety, inhibiting the secretion of glucagon during hyperglycemia, thus leading to reduction of the total insulin demand.

References
1. Diabetes. (1991) 40: 1005–1009. Release of amylin from perfused rat pancreas in response to glucose, arginine, β-hydroxybutyrate, and gliclazide. Inoue K., et al.
2. Diabetes. (1990) 39: 260–265. Induction of insulin resistance in vivo by amylin and calcitonin gene-related peptide. Molina J.M., et al.
3. Kidney Int. (2007) 72 (2): 213-8. Amylin deposition in the kidney of patients with diabetic nephropathy. Gong, et al.
4. Proc. Natl. Acad. Sci. USA. (1989) 86: 3127–3130. Rat amylin: cloning and tissue-specific expression in pancreatic islets. Leffert J.D., et al.
5. Nature. (1988) 335: 632–635. Pancreatic amylin and calcitonin gene-related peptide cause resistance to insulin in skeletal muscle in vitro. Leighton B. and Cooper G.J.S.
6. Proc. Natl. Acad. Sci. U.S.A.. (1987) 84: 3881–3885. Amyloid fibrils in human insulinoma and islets of Langerhans of the diabetic cat are derived from a neuropeptide-like protein also present in normal islet cells. Westermark, P., et al.
7. J. Biol. Chem. (1988) 263: 17243–17246. An islet amyloid peptide is derived from an 89-amino acid precursor by proteolytic processing. Sanke T., et al.
8. Diabetologia. (1978) 15 :417–421. The influence of amyloid deposits on the islet volume in maturity onset diabetes mellitus. Westermark P. and Wilander E.
9. Am J Physiol Endocrinol Metab. (2012) 302 (8): 924-31. Amylin improves the effect of leptin on insulin sensitivity in leptin-resistant diet-induced obese mice. Kusakabe T, et al.

  • Amylin EIA Kit
    Detailed protocol insert for this EIA kit for rat or mouse amylin.
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