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Angiotensin I EIA Kit Maximize

Angiotensin I EIA Kit

Angiotensin I EIA Kit (Human, Mouse, Rat, Canine)(EK-002-01) is a quantitative method for measuring amounts of Angiotensin I.

More details

EK-002-01

96 wells

£ 430.00

Angiotensin I EIA Kit

Specificity : Mouse, Rat, Human, Canine.
Sensitivity : 0.1ng/ml
Linear Range: 0.1-1.09 ng/ml
Assay Range : 0-25ng/ml
Size: 96 tests.

Reagents Supplied:
- Primary antibody (rabbit anti-peptide IgG)(1 vial).
- Standard peptide (1 vial).
- 96 well immunoplate (1 plate).
- Positive control (2 vials).
- 20x assay buffer concentrate (50ml).
- Streptavidin-horseradish peroxidase (SA-HRP)(30μl).
- Substrate solution (TMB) (12ml).
- Biotinylated peptide (1 vial).
- Acetate plate sealer (APS), (3 pieces).
- 2N HCl (15ml).
- Assay diagram (1 sheet).
- General protocol (1 book).

Intended Use:
Angiotensin I EIA Kit (Human, Mouse, Canine and Rat) is to be utilised for quantitative analysis of Angiotensin I levels in tissue homogenate, serum, CSF, plasma, any biological fluid and urine.

Assay Principle:
1. The microwell plate has been pre-coated with secondary antibody and also the non-specific binding sites have been blocked.
2. Secondary antibody is able to bind with the Fc fragment of the primary antibody, the Fab segment is competitively bound by both biotinylated peptides and the peptide standard or the targeted peptide in samples.
3. Biotinylated peptide can then interact with the SA-HRP, which then catalyzes the TMB substrate solution and hydrogen peroxide to form a blue coloured solution.
4. Reaction is stopped by adding hydrogen chloride, which forms a yellow coloured solution.
5. Intensity of the yellow solution is inversely proportional to the amount of the peptide in standard solutions or samples.
6. Unknown concentration in samples can then be determined using this angiotensin-I EIA assay by extrapolation from a standard curve.

Cross Reactivity:
Angiotensin I (Human, Mouse, Rat, Canine)  100%.
[Asn1, Val5, Asn9]-Angiotensin I (Salmon)  100%.
[Val5, Ser9]-Angiotensin I (Fowl)  100%.
[Val5, Asn9]-Angiotensin I (Bullfrog)  100%.
α-ANP  0%
Angiotensin II  0%.
Angiotensin III  0%.
BNP-32  0%.
Renin Substrate  0%.
Endothelin-1  0%.
[Arg8]-Vasopressin  0%.

Background:
Angiotensin-I is present within the blood and has been found to cause increased blood pressure, vasoconstriction and release of aldosterone from the adrenal cortex. It also has an vital role in the renin-angiotensin system. It is a powerful dipsogen and it is derived from precursor molecule angiotensinogen, which is a serum globulin that is made in the liver.
It is found to be formed by the action of renin on angiotensinogen. Renin is made by the kidneys in response to both:
(a). A decreased delivery of Na+ and Cl- to the macula densa.
(b). A decreased intra-renal blood pressure at the juxtaglomerular cells.

References
1. Am Soc Nephrol. (2011) 22 (7): 1240–1251. Podocyte COX-2 Exacerbates Diabetic Nephropathy by Increasing Podocyte (Pro)renin Receptor Expression. Huifang Cheng et al.
2. Am J Nephrol. (2008) 28 (1): 8-13. Effects of Resibufogenin in Experimental Hypertension. Danchuck et al.
3. Kidney Int. (2012) 82 (8): 857-66. Endothelial cell TGF-β receptor activation causes tacrolimus-induced renal arteriolar hyalinosis. Valorie L. Chiasson.
4. Asean Heart J; (2011) 19: 20-23. Different Plasma Angiotensin I levels in Indonesian Hypertensive Patients. Nani Hersunarti, et al.

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