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Angiotensin II EIA Kit Maximize

Angiotensin II EIA Kit

 Angiotensin II EIA kit (Human, Rat, Mouse, Canine)(EK-002-12) is a protocol to determine quantitatively Angiotensin-II concentrations.

More details

EK-002-12

96 wells

£ 430.00

Angiotensin II EIA Kit

Specificity : Rat, Human, Mouse, Canine.
Sensitivity : 0.09 ng/ml
Linear Range: 0.09-0.91 ng/ml
Assay Range : 0-25ng/ml
Size: 96 tests.

Reagents Supplied:
- Primary antibody (rabbit anti-peptide IgG)(1 vial).
- Standard peptide (1 vial).
- 96 well immunoplate (1 plate).
- Positive control (2 vials).
- 20x assay buffer concentrate (50ml).
- Streptavidin-horseradish peroxidase (SA-HRP)(30μl).
- Substrate solution (TMB) (12ml).
- Biotinylated peptide (1 vial).
- Acetate plate sealer (APS), (3 pieces).
- 2N HCl (15ml).
- Assay diagram (1 sheet).
- General protocol (1 book).

Intended Use:
Angiotensin-II EIA Kit (Human, Rat, Mouse or Canine)
is intended for in vitro quantitative study of Angiotensin-II amounts in any biological fluid, serum, plasma, tissue homogenate, urine and CSF.

Assay Principle:
1. Microwell plates have been pre-coated with secondary antibody and also the non-specific binding sites have been blocked.
2. Secondary antibody is able to bind with the Fc fragment of the primary antibody, the Fab segment is competitively bound by both biotinylated peptides and the peptide standard or the targeted peptide in samples.
3. Biotinylated peptide can then interact with the SA-HRP, which then catalyzes the TMB substrate solution and hydrogen peroxide to form a blue coloured solution.
4. Reaction is stopped by adding hydrogen chloride, which forms a yellow coloured solution.
5. Intensity of the yellow solution is inversely proportional to the amount of the peptide in standard solutions or samples.
6. Unknown concentration in samples can then be determined using this Angiotensin-II EIA assay by extrapolation from a standard curve.

Cross Reactivity:
Angiotensin II (Human, Mouse, Canine, Rat)  100%.
Angiotensin III (Human)  100%.
[Val5]-Angiotensin II (Human)  100%.
Angiotensin II (1-7) (Human)  0%.
Angiotensin I (Human)  1%.
Angiotensin I (1-9) (Human)  1%.
Renin Substrate  0%.
Angiotensin (1-7)  0%.
α-ANP (1-28)  0%.
[Sar1, Ile8]-Angiotensin II  0%.
[Arg8]-Vasopressin 0%.

Background:
Human Angiotensin-II is found to act as an autocrine, paracrine, intracrine hormone and endocrine. It has prothrombotic potential by its aggregation and adhesion of platelets. Itcan also cause vascular and cardiac muscle hypertrophy.  Increase the thirst sensation through the subfornical organ of the brain. Has a direct effect on the proximal tubules to increase Naion resorption.

References
1. Circulation. (2005) 111 (15): 1954-61. Insulin-mediated upregulation of the renin angiotensin system in human subcutaneous adipocytes is reduced by rosiglitazone. Harte et al.
2. Clin Exp Metastasis. (2012) 29 (8): 915-25. Diabetes-associated angiotensin activation enhances liver metastasis of colon cancer. Takasumi Shimomoto, et al.
3. BMC Pediatr. (2012) 12: 119. Association of body mass index, sagittal abdominal diameter and waist-hip ratio with cardiometabolic risk factors and adipocytokines in arab children and adolescents. Al-Attas OS, et al.
4. Hypertension. (2006) 48 (6): 1095-102. Local Renin Angiotensin Expression Regulates Human Mesenchymal Stem Cell Differentiation to Adipocytes. Matsushita et al.
5. Hypertension. (2012) 60: 285-287. Role of Copper Transport Protein Antioxidant 1 in Angiotensin II–Induced Hypertension: A Key Regulator of Extracellular Superoxide Dismutase. Kiyoshi Ozumi, et al.
6. Life Sci. (2006) 78 (26): 3066-73. Regulation of cardiac and renal mineralocorticoid receptor expression by captopril following myocardial infarction in rats. de Resende et al.
7. Biology of Reproduction. (2012) 86 (3): 68. Protein restriction to pregnant rats increases the plasma levels of angiotensin II and expression of angiotensin II receptors in uterine arteries. Gao H, et al.
8. Circulation. (2007) 115 (1): 67-75. Hemodynamic Modulation of Endocardial Thromboresistance. Kapur et al.
9. J Hum Hypertens. (2003) 17 (2): 133-8. Endothelin-1 and Vasopressin plasma levels are not associated with the insertion/deletion polymorphism of the human angiotensin 1-converting enzyme gene in patients with coronary artery disease. Al-Fakhri et al.
10. J Mol Cell Cardiol. (2006) 40 (5): 696-707. Nuclear accumulation of the AT1 receptor in a rat vascular smooth muscle cell line: effects upon signal transduction and cellular proliferation. Cook et al.

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