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Apelin-36 EIA Kit Maximize

Apelin-36 EIA Kit

Human Apelin-36 EIA Kit (EK-057-15)  is designed for quantitative detection of amount of human apelin 36.

More details

EK-057-15

96 wells

£ 480.00

Human Apelin 36 EIA Kit

Specificity : Human.
Sensitivity : 0.09ng/ml
Linear Range: 0.09-1.18ng/ml
Assay Range : 0-100ng/ml
Size: 96 tests.

Reagents Supplied:
- Apelin-36 Primary antibody (rabbit anti-peptide IgG)(1 vial).
- Apelin-36 Standard peptide (1 vial).
- 96 well immunoplate (1 plate).
- Apelin-36 Positive control (2 vials).
- 20x assay buffer concentrate (50ml).
- Streptavidin-horseradish peroxidase (SA-HRP)(30μl).
- Substrate solution (TMB) (12ml).
- Biotinylated peptide (1 vial).
- Acetate plate sealer (APS), (3 pieces).
- 2N HCl (15ml).
- Assay diagram (1 sheet).
- General protocol (1 book).

Intended Use:
Human Apelin 36 EIA kit
is procedure for quantitatively determining the levels of apelin-36 in any biological fluid, plasma, tissue homogenate, CSF, urine and serum.

Assay Principle:
1. Human Apelin-36 EIA has been pre-coated with secondary antibody and also the non-specific binding sites have been blocked.
2. Secondary antibody is able to bind with the Fc fragment of the primary antibody, the Fab segment is competitively bound by both biotinylated peptides and the peptide standard or the targeted peptide in samples.
3. Biotinylated peptide can then interact with the SA-HRP, which then catalyzes the TMB substrate solution and hydrogen peroxide to form a blue coloured solution.
4. Reaction is stopped by adding hydrogen chloride, which forms a yellow coloured solution.
5. Intensity of the yellow solution is inversely proportional to the amount of the peptide in standard solutions or samples.
6. Unknown concentration of apelin 36 in samples can then be determined using this Apelin-36 EIA assay by extrapolation from a standard curve.

Cross Reactivity:
Apelin-36 (Human) 100%.
Apelin-36 (Rat) 100%.
Apelin-12 (Human) 100%.
Apelin-13 (Human) 100%.
Alpha ANP  0%.
ANP (25-56) (N-ANP) 0%.
ADM-52  0%.
BNP-32  0%.
CNP-22 0%.
Ghrelin  0%.
ET-1  0%.
Bradykinin  0%.

Background:
Apelin 36 levels are increased in left ventricles of patients with chronic heart failure. An endogenous ligand for the G-protein-coupled APJ receptor. It is widely expressed in various organs such as the heart, gastrointestinal tract, kidney, lung, adipose tissue, endothelium, brain, adrenal glands and human plasma. Also, a potent stimulators of cardiac contractility, playing a vital part in cardiac tissue remodeling. It functions as a mediator of the cardiovascular control, which includes blood flow and blood pressure.

References
1. Peptides. (2012) 37 (2): 233-9. The bioactive peptides salusins and apelin-36 are produced in human arterial and venous tissues and the changes of their levels during cardiopulmonary bypass. Suna Aydin, et al.
2. Ren Fail. 2012;34(9):1068-73. Volume Status in Patients on Peritoneal Dialysis: The Role of Apelin and Bio-Impedance Spectroscopy. Rumeyza Kazancioglu, et al.
3. Eur J Endocrinol (2008) 158: 905-910. Effect of hypocaloric diet-induced weight loss in obese women on plasma apelin and adipose tissue expression of apelin and APJ. Isabelle Castan-Laurel et al.,
4. Steroids. (2012) 77 (13): 1352-8. Serum asymmetric dimethylarginine, apelin, and tumor necrosis factor-α levels in non-obese women with polycystic ovary syndrome. Young Sik Choi, et al.
5. In Vivo (2002) 16 (5): 333-6. Apelin expression in normal human tissues. De Falco et al.

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