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ANP Alpha (1-28) EIA Kit Maximize

ANP Alpha (1-28) EIA Kit

ANP (1-28) Alpha EIA Kit (Human, Canine, Ovine, Porcine)(EK-005-06) an in-vitro quantitative procedure designed to analyse  levels alpha atrial natriuretic peptide alpha (ANP-alpha).

More details

EK-005-06

96 wells

£ 430.00

ANP Alpha (1-28) EIA Kit

Specificity : Human, Ovine, canine, Porcine
Sensitivity : 0.12 ng/ml
Linear Range: 0.12-1.32ng/ml
Assay Range : 0-25 ng/ml
Size: 96 tests.

Reagents Supplied:
- Primary antibody (rabbit anti-peptide IgG)(1 vial).
- Standard peptide (1 vial).
- 96 well immunoplate (1 plate).
- Positive control (2 vials).
- 20x assay buffer concentrate (50ml).
- Streptavidin-horseradish peroxidase (SA-HRP)(30μl).
- Substrate solution (TMB) (12ml).
- Biotinylated peptide (1 vial).
- Acetate plate sealer (APS), (3 pieces).
- 2N HCl (15ml).
- Assay diagram (1 sheet).
- General protocol (1 book).

Intended Use:
ANP Alpha EIA kit (Human, Canone, Ovine, Porcine) is an in vitro quantitative method for measuring levels of Alpha Atrial Natriuretic Peptide (alpha-ANP)(1-28) in tissue homogenate, any biological fluid, serum, plasma, CSF and urine.

Assay Principle:
1. ANP Alpha EIA kit has been pre-coated with secondary antibody and also the non-specific binding sites have been blocked.
2. Secondary antibody is able to bind with the Fc fragment of the primary antibody, the Fab segment is competitively bound by both biotinylated peptides and the peptide standard or the targeted peptide in samples.
3. Biotinylated peptide can then interact with the SA-HRP, which then catalyzes the TMB substrate solution and hydrogen peroxide to form a blue coloured solution.
4. Reaction is stopped by adding hydrogen chloride, which forms a yellow coloured solution.
5. Intensity of the yellow solution is inversely proportional to the amount of the peptide in standard solutions or samples.
6. Unknown concentration of ANP alpha in samples can then be determined with this ANP Alpha EIA kit by extrapolation from a standard curve.

Cross Reactivity:
alpha-ANP (1-28) (Human, Bovine,  Porcine, Canine) 100%.
beta ANP (Human) 100%.
ANP (Rat) 0%.
BNP-26 (Porcine) 0.1%.
Endothelin-1  0%.
BNP-32 (Human)  0%.
BNP-45 (Rat)  0%.
CNP-22  0%.

Background:
Atrial natriuretic peptide alpha (ANP) alpha is a 28 amino acid peptide with a 17 amino acid ring in the middle of the molecule. It is a polypeptide hormone that has a vital role in the homeostatic control of body water, adiposity and sodium. It is found to be released by atrial myocytes, muscle cells in the atria of the heart, in response to high blood pressure.It's primary function is to lower the sodium, water and adipose loads on the circulatory system, thereby reducing blood pressure.

References
1. Türk Kardiyol Dern Arş (2007) 35: 412-416. The effect of carvedilol on big endothelin, atrial and brain natriuretic peptide levels in patients with congestive heart failure. Sevimli et al.
2. Hypertens Res. (2008) 31 (4): 767-74. Arterial stiffness, physical activity, and atrial natriuretic Peptide gene polymorphism in older subjects. Iemitsu et al.
3. Clin Rheumatol. (2008) 27 (9): 1153-8. Serum levels of natriuretic peptides in patients with Behcet's disease. Yagci et al.

  • Alpha ANP EIA Kit
    Detailed protocol insert for this alpha ANP EIA kit (atrial natriuretic peptide).
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