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ANP EIA Kit (Mouse, Rat) Maximize

ANP EIA Kit (Mouse, Rat)

ANP EIA Kit (Mouse, Rat) (EK-005-24) is designed to be utilised for quantitatively detecting in-vitro concentrations of atrial natriuretic peptide (ANP).

More details

EK-005-24

96 wells

£ 430.00

ANP EIA Kit

Specificity : Rat or Mouse
Sensitivity : 0.2 ng/ml
Linear Range: 0.2-5.5ng/ml
Assay Range : 0-100 ng/ml
Size: 96 tests.

Reagents Supplied:
- Primary antibody (rabbit anti-peptide IgG)(1 vial).
- Standard peptide (1 vial).
- 96 well immunoplate (1 plate).
- Positive control (2 vials).
- 20x assay buffer concentrate (50ml).
- Streptavidin-horseradish peroxidase (SA-HRP)(30μl).
- Substrate solution (TMB) (12ml).
- Biotinylated peptide (1 vial).
- Acetate plate sealer (APS), (3 pieces).
- 2N HCl (15ml).
- Assay diagram (1 sheet).
- General protocol (1 book).

Intended Use:
ANP EIA kit
(Mouse, Rat)can be used for in vitro quantitative analysis of atrial natriuretic peptide (ANP) levels in plasma, tissue homogenate, serum, any biological fluid, CSF and urine.

Assay Principle:
1. This ANP EIA kit has been pre-coated with secondary antibody and also the non-specific binding sites have been blocked.
2. Secondary antibody is able to bind with the Fc fragment of the primary antibody, the Fab segment is competitively bound by both biotinylated peptides and the peptide standard or the targeted peptide in samples.
3. Biotinylated peptide can then interact with the SA-HRP, which then catalyzes the TMB substrate solution and hydrogen peroxide to form a blue coloured solution.
4. Reaction is stopped by adding hydrogen chloride, which forms a yellow coloured solution.
5. Intensity of the yellow solution is inversely proportional to the amount of the peptide in standard solutions or samples.
6. Unknown concentration of mouse or rat ANP in samples can then be determined using this ANP EIA assay by extrapolation from a standard curve.

Cross Reactivity:
ANP (1-28)(Rat) 100%
Beta ANP (1-28)(Human) 100%.
Alpha ANP (1-28)(Human, Canine, Porcine, Bovine) 100%.
Alpha ANP (8-33)(Rat) 100%.
Urodilatin 100%.
BNP-26 (Porcine)  0.1%.
BNP-45  0%.
BNP-32  0%.
Endothelin-1  0%.
CNP-22  0%.

Background:
Atrial natriuretic peptide (ANP) s a polypeptide hormone that has a vital role in the homeostatic control of body water, adiposity and sodium. It is a 28 amino acid peptide with a 17 amino acid ring in the middle of the molecule that is released by atrial myocytes, muscle cells in the atria of the heart, in response to high blood pressure. It functions to lower the sodium, water and adipose loads on the circulatory system, thereby reducing blood pressure.

References
1. Cardiovasc Res. (2007) 75 (1): 40-50. S100A4 is upregulated in injured myocardium and promotes growth and survival of cardiac myocytes. Schneider et al.
2. Am J Physiol Heart Circ Physiol. (2009) 296 (3): 655-61. Atrial natriuretic peptide increases inflammation, infarct size, and mortality after experimental coronary occlusion. Houng et al.
3. Endocrinology. (2007) 148 (5): 2075-84. Alteration of Glucose Homeostasis in V1a Vasopressin Receptor-Deficient Mice. Aoyagi et al.
4. Circulation. (2007) 115 (1): 67-75. Hemodynamic Modulation of Endocardial Thromboresistance. Kapur et al.

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