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BNP-32 EIA Kit Maximize

BNP-32 EIA Kit

Porcine BNP-32 EIA Kit (EK-011-10) an in-vitro quantitative method to measure porcine brain natriuretic peptide-32 (BNP-32) levels.

More details

EK-011-10

96 wells

£ 430.00

Porcine BNP-32 EIA Kit

Specificity : Porcine
Sensitivity : 0.45 ng/ml
Linear Range: 0.45-6.3 ng/ml
Assay Range : 0-100 ng/ml
Size: 96 tests.

Reagents Supplied:
- BNP-32 Primary antibody (rabbit anti-peptide IgG)(1 vial).
- BNP-32 Standard peptide (1 vial).
- 96 well immunoplate (1 plate).
- BNP-32 Positive control (2 vials).
- 20x assay buffer concentrate (50ml).
- Streptavidin-horseradish peroxidase (SA-HRP)(30μl).
- Substrate solution (TMB) (12ml).
- Biotinylated peptide (1 vial).
- Acetate plate sealer (APS), (3 pieces).
- 2N HCl (15ml).
- Assay diagram (1 sheet).
- General protocol (1 book).

Intended Use:
Porcine BNP-32 EIA kit can be used for in vitro quantitative determination of brain natriuretic peptide-32 levels in plasma, serum, culture media, tissue homogenate, CSF, urine and any biological fluid.

Assay Principle:
1. The microwell plate has been pre-coated with secondary antibody and also the non-specific binding sites have been blocked.
2. Secondary antibody is able to bind with the Fc fragment of the primary antibody, the Fab segment is competitively bound by both biotinylated peptides and the peptide standard or the targeted peptide in samples.
3. Biotinylated peptide can then interact with the SA-HRP, which then catalyzes the TMB substrate solution and hydrogen peroxide to form a blue coloured solution.
4. Reaction is stopped by adding hydrogen chloride, which forms a yellow coloured solution.
5. Intensity of the yellow solution is inversely proportional to the amount of the peptide in standard solutions or samples.
6. Unknown concentration of BNP-32 in samples can then be determined using this porcine BNP-32 EIA assay by extrapolation from a standard curve.

Cross Reactivity:
BNP-32 (Porcine)  100%.
BNP-26 (Porcine)  100%.
BNP-32 (Rat, Human)  0%.
BNP-45  0%.
Alpha ANP (1-28)  0%.

Background:
Brain natriuretic peptide was first discovered in extracts of porcine brain, whereas within humans it is found to be made in the cardiac ventricles. It is a 32 a.a. polypeptide has been found to be secreted by the ventricles of heart when there is excessive stretching of myocytes. It is release alongside an NT-proBNP (76 amino acids amino terminal fragment). Elevated levels of BNP 32 and/or NT-proBNP within the blood are useful markers in the diagnosis of heart failure and acute congestive heart failure (CHF).

References
1. Circulation. (2007) 116 (4): 399-410. ß1-Adrenergic Receptor Autoantibodies Mediate Dilated Cardiomyopathy by Agonistically Inducing Cardiomyocyte Apoptosis. Jane-wit et al.
2. Cardiovasc Pathol. (2007) 16 (2): 79-84. Plasma brain natriuretic peptide correlates with infarct size but not with subsequent remodeling in the rat heart. Maczewski et al.
3. J Mol Cell Cardiol. (2006) 41 (5): 798-806. Down-regulation of cardiac apelin system in hypertrophied and failing hearts: Possible role of angiotensin II-angiotensin type 1 receptor system. Iwanaga et al.
4. Circulation. (2007) 115 (1): 67-75. Hemodynamic Modulation of Endocardial Thromboresistance. Kapur et al.

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