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BNP-32 EIA Kit Maximize

BNP-32 EIA Kit

Human BNP-32 EIA Kit (EK-011-03) procedure designed to analyse quantitatively the concentration of human brain natriuretic peptide (BNP 32).

More details

EK-011-03

96 wells

£ 430.00

Human BNP-32 EIA Kit

Specificity : Human
Sensitivity : 0.26ng/ml
Linear Range: 0.26-3.33 ng/ml
Assay Range : 0-100 ng/ml
Size: 96 tests.

Reagents Supplied:
- BNP-32 Primary antibody (rabbit anti-peptide IgG)(1 vial).
- BNP-32 Standard peptide (1 vial).
- 96 well immunoplate (1 plate).
- BNP-32 Positive control (2 vials).
- 20x assay buffer concentrate (50ml).
- Streptavidin-horseradish peroxidase (SA-HRP)(30μl).
- Substrate solution (TMB) (12ml).
- Biotinylated peptide (1 vial).
- Acetate plate sealer (APS), (3 pieces).
- 2N HCl (15ml).
- Assay diagram (1 sheet).
- General protocol (1 book).

Intended Use:
Human BNP-32 EIA kit is a method for quantitatively analysing amounts of brain natriuretic peptide levels in plasma, culture media, serum, tissue homogenate, urine, CSF and any biological fluid.

Assay Principle:
1. Pre-coated microwells with secondary antibody and also the non-specific binding sites have been blocked.
2. Secondary antibody is able to bind with the Fc fragment of the primary antibody, the Fab segment is competitively bound by both biotinylated peptides and the peptide standard or the targeted peptide in samples.
3. Biotinylated peptide can then interact with the SA-HRP, which then catalyzes the TMB substrate solution and hydrogen peroxide to form a blue coloured solution.
4. Reaction is stopped by adding hydrogen chloride, which forms a yellow coloured solution.
5. Intensity of the yellow solution is inversely proportional to the amount of the peptide in standard solutions or samples.
6. Unknown concentration of BNP-32 in samples can then be determined using this Human BNP-32 EIA assay by extrapolation from a standard curve.

Cross Reactivity:
BNP-32 (Human)  100%.
BNP-32 (Rat)  0.04%.
BNP-26 (Porcine)  0%.
BNP-45 (Rat)  0%.
Angiotensin II  0%
Alpha ANP (1-28)  0%.
Endothelin-1  0%.
VIP  0%.

Background:
Brain natriuretic peptide was first discovered in extracts of porcine brain, whereas within humans it is found to be made in the cardiac ventricles. Is a 32 a.a. polypeptide has been found to be secreted by the ventricles of heart when there is excessive stretching of myocytes. It is release alongside an NT-proBNP (76 amino acids amino terminal fragment). Elevated levels of BNP-32 and/or NT-proBNP within the blood are useful markers in the diagnosis of heart failure and acute congestive heart failure (CHF).

References
1. Türk Kardiyol Dern Arş (2007) 35: 412-416. The effect of carvedilol on big endothelin, atrial and brain natriuretic peptide levels in patients with congestive heart failure. Sevimli et al.
2. Clin Rheumatol. (2008) 27 (9): 1153-8. Serum levels of natriuretic peptides in patients with Behcet's disease. Yagci et al.
3. Pediatrics. (2008) 121 (5): 1208-14. Increased morning brain natriuretic peptide levels in children with nocturnal enuresis and sleep-disordered breathing: a community-based study. Sans Capdevila et al.
4. Int J Cardiol. (2008) 123 (2): 102-7. The effects of short term statin treatment on left ventricular function and inflammatory markers in patients with chronic heart failure. Gürgün et al.
5. Clin Chem. (2011) 57 (6): 864-73. Secretion of glycosylated pro-B-type natriuretic peptide from normal cardiomyocytes. Tonne JM et al.

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