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5-alpha DHT ELISA Kit Maximize

5-alpha DHT ELISA Kit

Human 5alpha DHT ELISA Kit (DB52021) is a quantitative protocol intended to determine in-vitro levels of human 5 alpha Dihydrotestosterone.

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DB52021

96 wells

£ 350.00

Human 5-alpha DHT ELISA Kit

Specificity : Human, serum
Sensitivity : 6.0 pg/ml
Assay Range : 25-2500 pg/ml
Size: 96 tests

Specificity : Human
Sensitivity : 0.8 ng/mL.
Assay Range : 0.8 - 50 ng/mL
Size: 96 tests

Reagents Supplied:
- Microtiter plate  [96 Wells]
- 5 alpha DHT HRP Enzyme Conjugate, Conc. (100 x)  [0.25ml]
- 5 alpha DHT Standard A-F [1X 2ml & 5X 1ml]
- 5 alpha DHT Controls [2x 1ml]
- TMB Substrate [18ml]
- Stopping Solution  [18ml]
- Washing Buffer Conc. 10x  [2x 50ml]
- Assay Buffer  [17ml]

Key Features:
[1]. Method:  ELISA
[2]. Kit Size: 96 Wells.
[3]. Standard Range:  25 – 2500 pg/ml
[4]. Incubation Time: 1x 1 h, 1x 10 min.
[5]. Substrate: TMB 450nm
[6]. Volume/Specimen: 50μl serum (no extraction)
[7].Regulatory Status: IVD, CE, CDN, EU

Intended Use:
Human 5-alpha DHT ELISA kit a procedure for in-vitro quantitatively diagnosing levels of human 5 alpha Dihydrotestosterone using human serum.

Expected Values:
The below normal values were obtained.
[1]. Females Premenopausal  [24-368pg/ml].
[2]. Females Postmenopausal [10-181pg/ml].
[3]. Males  [250-990pg/ml].

Specificity [Cross Reactivity (%)]:
- Dihydrotestosterone (DHT)  [100%].
- Testosterone  [8.7%]
- 5 beta Dihydrotestosterone (DHT)  [2.0%]
- Androstenedione  [0.2%]
- Less than 0.01% for the following steroids: Progesterone, Estriol, 17beta Estradiol, Pregnenolone, Dehydroepiandrosterone Sulfate, 17-OH Progesterone, Estrone and Cortisol.

References
1. Ann. Clin. Biochem. (1986) 23:590. Solitary androgens in hirsutism: Are they of use in routine evaluation. Wang, C., et al.
2. Clinica Cemica Alta (1977) 80:171. Study of the binding of dihydrotestosterone, testosterone and oestradiol with sex hormone binding globulin. Mean, F., et al.
3. J. Clin. Endocr. Metab. (1981) 53:58. Transport of Steroid Hormones: Binding of 21 endogenous steroids to both SHBG and CBG in human plasma. Dunn, J.F., et al.
4. Medical Laboratory Sciences, (1980) 37:31. A simple chromatographic method for the radioimmunoassay of four androgenic steroids. Bassett, R.M.
5. Clin. Endocr. (1976) 82:380. Radioimmunoassay of plasma dihydrotestosterone in normal and hypogonadal men. Pazzagli, M., et al.
6. Clin. Chemistry (1999) 45:7. Human anti-animal antibody interferences in immunological assays. Kricka, L.J.
7. Clinical Endocrinology, (1983) 18:447. Plasma and salivary androstenedione and dihydrotestosterone in women with hyperandrogenism. Baxendale, P.M., et al.
8. J. Clin. Endocr. (1970) 31:362. Dihydrotestosterone in human peripheral plasma. Ito, T., et al.
9. J. Endocrinol. (1980) 87:450. Relationship of 5ßdihydrotestosterone and 5 alpha dihydrotestosterone to testosterone in health and disease. Wakelin, K., et al.
10. J. Clin. Endocr. Metab. (1977) 45:16. The simultaneous radioimmunoassay of seven steroids in human spermatic and peripheral venous blood. Hammond, G.L., et al.
11. Endocr. Rev. (1987) 8:1-28. The biological actions of androgens. Mooradian, A.D., et al.

  • 5alpha DHT ELISA
    Detailed protocol insert for human 5 alpha Dihydrotestosterone ELISA Kit
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