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11-keto Testosterone EIA Kit Maximize

11-keto Testosterone EIA Kit

11-keto Testosterone EIA Kit (CM582751) is a quantitative method to detect concentrations of 11 keto testosterone in a range of different samples.

More details

CM582751

96 wells

£ 480.00

11-Keto Testosterone EIA Kit

Specificity : Human
Sensitivity : 1.3 pg/ml
Assay Range : 7.8 – 1000 pg/mL
Size: 96 tests

Reagents Supplied:
- Mouse Anti-Rabbit IgG Coated Plate  [96 wells]
- EIA Antiserum  [100 dtn]
- AChE Tracer  100 dtn]
- 11-keto Testosterone Standard  [1 vial]
- EIA Buffer Concentrate (10X)  [2x 10ml]
- Wash Buffer Concentrate (100X)  [5ml]
- Tween 20  [3ml]
- Plate Cover  [1x]
- Ellman’s Reagent  [100 dtn]
- EIA Tracer Dye  [1 vial]
- EIA Antiserum Dye  [1 vial]

Key Features:
[1]. Method:  EIA
[2]. Kit Size: 96 Wells [12x8].
[3]. Standard Range:  7.8 – 1000 pg/mL.
[4]. Incubation Time: 1x 18 h, 1x 1 h.
[5]. Substrate: ER 412nm
[6]. Volume/Specimen: 50μl serum or plasma (extraction)
[7].Regulatory Status:  Research Use.

Intended Use:
This EIA for 11-keto Testosterone is competitive assay procedure to quantitatively determine in vitro levels of 11-keto testosterone in plasma or other sample matrices.

Principle:
This 11-keto Testosterone assay is based on the competition between 11-KT and a 11-KT-acetylcholinesterase (AChE) conjugate (11-KT tracer) for a limited number of 11-KT-specific rabbit antiserum binding sites. Because the concentration of the 11-KT tracer is held constant while the concentration of 11-KT varies, the amount of 11-KT racer that is able to bind to the rabbit antiserum will be inversely proportional to the concentration of 11-KT in the well. This rabbit antiserum-11-KT complex binds to the mouse monoclonal anti-rabbit IgG antibody that has been previously attached to the well. The plate is washed to remove any unbound reagents and then Ellman’s Reagent is added to the well. The product that is formed has a distinct yellow color and the concentration determined spectrophotometrically, is proportional to the amount of 11-KT tracer bound to the well, which is inversely proportional to the amount of free 11-KT present in the well during incubation.

Specificity [Cross Reactivity (%)]:
- 11 keto Testosterone  [100%]
- Testosterone  [0.01%]
- 4-Androsten-11b,17b-diol-3-one [0.01%]
- 5a-Androsten-3b,17b-diol  [below 0.01%]
- 5a-Androstan-17b-ol-3-one  [below 0.01%]

References
1. Anal. Chem. (1985) 57,1170-1173. Enzyme immunoassays of eicosanoids using acetylcholinesterase as label: An alternative to radioimmunoassay. Pradelles, P., Grassi, J., and Maclouf, J.A.
2. Dicentrarchus labrax L. Gen. Comp. Endocrinol. (1990) 78, 361-373. Seasonal changes in plasma levels of gonadal steroids of sea bass. Prat, F., et al.
3. J. Clin. Immunoassay (1992) 15, 116-120 (1992). J. Interference in enzyme immunoassays. Maxey, K.M., Maddipati, K.R., and Birkmeier,
4. Plenum Press, Rockville, (1987) 355-364. Development of enzyme-immunoassay techniques for the measurement of eicosanoids, Chapter 5, in Prostaglandin and Lipid Metabolism in Radiation Injury. Walden, T.L., Jr. and Hughes, H.N., editors, Maclouf, J., Grassi, J., and Pradelles, P.
5. Gen. Comp. Endocrinol. (2000) 118, 450-460 (2000). Intermale competition in sexually mature arctic charr: effects on brain monoamines, endocrine stress responses, sex hormone levels, and behaviour. Elofsson, U.O.E., et al.

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