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17-beta Estradiol ELISA Kit Maximize

17-beta Estradiol ELISA Kit

Human 17-beta Estradiol ELISA Kit (RE52041) is intended for quantitatively analysing in-vitro concentrations of human 17beta Estradiol.

More details

RE52041

96 wells

£ 350.00

Human 17-beta Estradiol ELISA Kit

Specificity : Human
Sensitivity : 9.714 pg/mL.
Assay Range : 25 – 2000 pg/mL.
Size: 96 tests

Reagents Supplied:
- Microtiterwells (12x8 break apart strips) [96 wells]
- Standard (Standard 0-6) [7x 1 ml]
- Enzyme Conjugate  [25 ml]
- TMB Substrate Solution [14 ml]
- Stopping Solution  [14 ml]
- Washing Solution  [30 ml] (40X concentrated);

Key Features:
[1]. Method:  ELISA
[2]. Kit Size: 96 Wells [12x8].
[3]. Standard Range:  25 – 2000 pg/mL
[4]. Incubation Time: 1x 2 h, 1x 15 min.
[5]. Substrate: TMB 450nm
[6]. Volume/Specimen: 25μl serum and plasma
[7].Regulatory Status:  CE, EU

Intended Use:
This human 17-beta Estradiol ELISA is a protocol designed for in vitro quantitative diagnostic determination of human 17beta Estradiol levels in serum or plasma.

Principle:
This 17-beta estradiol is a solid phase competitive based enzyme-linked immunosorbent assay. Microtiter wells present are coated with a polyclonal rabbit antibody directed towards an antigenic site on the estradiol molecule. Endogenous estradiol obtained from patients will compete with the estradiol-horseradish peroxidase conjugate for binding to the coated antibody. After incubation the unbound conjugate is washed off. The amount of bound peroxidase conjugate is inversely proportional to the concentration of estradiol in the sample. Following the addition of the substrate solution, the intensity of colour developed is inversely proportional to the concentration of estradiol in the patient sample.

Expected Values:
[a]. Males : 10 - 36 pg/mL  [5 – 95% Percentile]
[b]. Females (pre-menopausal):  13 - 191 pg/mL  [5 – 95% Percentile]
[c]. Females (post-menopausal):  11 - 65 pg/mL  [5 – 95% Percentile]  
* Each lab. is recommended to establishes its own normal or abnormal values.

Interfering Substances:
Bilirubin (under 0.5 mg/mL), Haemoglobin (under 4 mg/mL) or Triglyceride (under 30 mg/mL) have no influence on the assay results.

Specificity [Cross Reactivity (%)]:
- 17beta Estradiol [100%]
- Estriol [0.05%]
- Estrone  [0.2%]
- 0% for the following compounds : 11-Deoxycortisol, Androstenedione, 21-Deoxycortisol, Androsterone, Dihydrotestosterone, Corticsterone, Dihydroepiandrosterone, Cortisone, 20-Dihydroprogesterone, Epiandrosterone, 11-Hydroxyprogesterone, 16-Epiestriol, 17alpha-Hydroxyprogesterone, Estradiol-3-sulfate, 17 alpha-Pregnenolone, Estradiol-3-glucoronide, 17 alpha-Progesterone, Estradiol-17 alpha, Pregnanediol, Pregnanetriol, Estriol-16-glucoronide, Pregnenolone, Progesterone, Estrone-3-sulfate, Testosterone, Dehydroepiandrosterone

References
1. Fertil. Steril. (1982) 37: 348-54. Correlation between oestradiol and progesterone in cycles with luteal phase deficiency. Goldstein, D., et al.
2. Rec. Prog. Horm. Res. (1982) 38:457 – 510. The serum transport of steroid hormones. Siiteri, P.K., et al.
3. Ann. Clin. Biochem. (1988) 25:466-483. Estradiol assays: applications and guidelines for the provision of clinical biochemistry service. Ratcliff, W.A.., Carter, G.D.
4. Endocr. (1978) 8:149-80. Abnormalities of gonadal function in men. Odell, W.D. and Swerdloff, R.D.
5. J. Clin. Endocrinol. Metab. (1980) 51:1407 – 11. Steroid biosyntheses by isolated human ovarian follicular cells in vitro. Tsang, B.K., et al.
6. J.Clin. Endicrinol. Metab. (1981) 35: 443-47. Binding of steroids by proteins in follicular fluid of the human ovary. Martin, B., et al.
7., Human Reproduction (1987) 2: 325-28. Pregnancy rate in relation to number of cleaved eggs replaced after in vitro-fertilisation of stimulating cycles monitored by serum levels of oestradiol and progesterone as sole index. Wramsby, H., Sundstorm, P- and Leidholm, P.
8. Cancer (1977) 39:2716 26. The role of hormones in the etiology of human breast cancer. Kirschner, M.A.

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