Sub Categories

Search

Newsletter

Androstenedione ELISA Kit Maximize

Androstenedione ELISA Kit

Human Androstenedione ELISA Kit (Cat. No.#  DB52161) method intended for directly measuring quantitatively in vitro diagnostic concentrations of androstenedione in human serum.

More details

DB52161

96 wells

£ 350.00

Human Androstenedione ELISA Kit

Specificity : Human, serum
Sensitivity : 0.04ng/ml
Assay Range : 0.1-10ng/ml
Size: 96 tests

Reagents Supplied:
- Microtiterwells (12x8 break apart strips) [96 wells]
- Androstenedione Enzyme Conjugate Concentrate (50 x) [0.3ml]
- Androstenedione Standards (Standard A-F) [1x 2ml and 5x 1ml]
- Androstenedione Controls [2x 1ml]
- TMB Substrate Solution [18ml]
- TMB Stop Solution  [8ml]
- Washing Solution  [2x 50ml] (10X concentrated)
- Assay Buffer  [17ml]

Key Features:
[1]. Method:  ELISA
[2]. Kit Size: 96 Wells [12x8].
[3]. Standard Range:  0.1 – 10 ng/mL
[4]. Incubation Time: 1x 1 h, 1x 15 min.
[5]. Substrate: TMB 450nm
[6]. Volume/Specimen: 25μl serum
[7].Regulatory Status:  CE, EU

Intended Use:
Human androstenedione ELISA kit is a procedure for directly analysing invitro quantitative levels of androstenedione present within human serum samples.

Expected Values:
[a]. 20 Males : 20ng/ml [Mean] and 0.4-3.5ng/ml [Range].
[b]. 20 Males : 1.4ng/ml [Mean] and 0.3-2.4ng/ml [Range].
* Each lab. is recommended to establishes its own normal values when using this androstenedione ELISA kit .

Specificity [Cross Reactivity (%)]:
- Androstenedione  [100%]
- DHEA  [1.8%]
- Testosterone  [0.2%]
- Less than 0.1% for the following substances: Estrone, Estradiol, Progesterone, 17-OH-Progesterone, 5 alpha-DHT, Cortisol, DHEA-S.

References
1. Clin. Chem. Acta. (1979) 94:327. Lejeune-Lenair, C., et al.
2. J. Steroid Biochem. (1980) 13:89. Berel, E., et al.
3. Am. Clin. Biochem. (1988) 23:354. Swinkles, L.M., et al.
4. Gynecol Obstet Invest (1995) 40:139-140. Check, J.H., et al,
5. Acta Endocrinol.(1974) 76:597. Hennam, J.F., et al.
6. J. Clin.Chem.Clin.Biochem. (1982) 20:761. Putz, Z., et al.
7. Scand.J.Clin.Lab.Invest. (1983) 43:301. Hummer, L., et al.
8. Endocrinology (1975) 97:787. Schandader, B.D., et al.
9. J. Steroid Biochem. (1980) 12:509. Pizarro, M.A., et al.,
10. J. Clin. Endo. Metabo. (1973) 36:475. Judd, H.L., et al.
11. J. Steroid Biochem (1975) 6:283. Bermudez, J.A., et al.
12. Steroids (1973) 21:111. Thorneycroft, I.H., et al.
13. Steroids (1977) 29:715. Parker, L.N., et al.
14. J. Steroid Biochem (1978) 9:771. Hampl, R., et al.
15. Steroids (1974) 24:793. Rao, PN., et al.
16. Clin Chem. (1987) 33:300. Slaats, EH., et al.

Special Offers