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25-OH Vitamin D total ELISA Kit Maximize

25-OH Vitamin D total ELISA Kit

Human 25-OH Vitamin D ELISA Kit (MG59061) is an in vitro method designed to measure quantitatively the concentrations of human 25-hydroxyvitamin D2 and D3 (total) present in serum samples.

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96 wells

£ 580.00

Human 25-OH Vitamin D ELISA Kit

Specificity : Human, serum
Sensitivity : 2.81ng/ml
Assay Range : 5.3 – 133.0ng/ml
Size: 96 tests

Reagents Supplied:
- Microtiterwells (12x8 break apart strips) [96 wells]
- Calibrator 0  [1 vial lyophilised]
- Calibrators 1-5 [5 vial lyophilised]
- Controls [2 vial lyophilised]
- Incubation Buffer  [20ml]
- Concentrated Conjugate  [0.4ml]
- Conjugate Buffer  [30ml]
- Concentrated HRP  [0.2ml]
- Wash Solution [10ml] (200X concentrated)
- TMB Substrate Solution  [12ml]
- TMB Stop Solution  [12ml]

Key Features:
[1]. Method:  ELISA
[2]. Kit Size: 96 Wells [12x8].
[3]. Standard Range:  5.3 – 133.0ng/ml.
[4]. Incubation Time: 1x 2 h , 1x 30 min , 1x 15 min.
[5]. Substrate: TMB 450nm.
[6]. Volume/Specimen: 50μl serum.
[7].Regulatory Status:  CE , EU.

Intended Use:
Human 25-OH Vitamin D ELISA Kit is to be used for quantitative in vitro determination of total 25-hydroxy vitamin D2 and D3 amounts in serum.

Expected Values:
Current studies indicate that the below ranges can be used for the classification of 25 OH Vitamin D status or levels:
(a). Deficient  [below 10ng/ml]
(b). Insufficient  [10-29ng/ml]
(c). Sufficient  [30-100ng/ml]
(d). Potential Toxicity   [above 100ng/ml].

Specificity [Cross Reactivity %]:
- 25-OH Vitamin D3 at 10ng/mL  [100%]
- 25-OH Vitamin D2 at 10ng/mL  [83%]
- 1,25-(OH)2 Vitamin D3 at 200ng/mL  [20%]
- 1,25-(OH)2 Vitamin D2 at 690ng/mL  [1.9%]
- Vitamin D3 at 200ng/mL  [2.9%]
- Vitamin D2 at 200ng/mL  [1.3%]
- 24,25-(OH)2-Vitamin D3 at 20ng/mL  [>100%]
- 25,26-(OH)2-Vitamin D3 at 4ng/mL  [>100%]
- 3-epi-25-OH-Vitamin D3 at 20μg/mL  [0.1%]

1. Ann. Epidemiol., (2009) 19:73-78. Vitamin-D Status: Measurement, Interpretation, and Clinical Application. Holicks MF.
2. Am. J. Clin. Nutr. (2008) 87(suppl):1087S-91S. Blood biomarkers of Vitamin D status. Zerwekh JE.
3. Osteoporos. Int., (2000) 11:553-555. Vitamin-D: how much do we need, and how much is too much. Heaney RP.
4. Clinical Laboratory International , November (2010) Vol.34: 28-30. The problem of an optimal target level for 25-Hydroxyvitamin D, the test for vitamin D nutritional status. Tahan NM and Vieth R.
5. J. Clin. Invest., (2006) 116:2062-2072. Resurrection of Vitamin-D deficiency and rickets. Holick MF.
6. Am. J. Clin. Nutr., (2006) 84:18-28. Estimation of optimal serum concentrations of 25-hydroxyvitamin D for multiple health outcomes. Bischoff-Ferrari HA., et al.
7. N. Engl. J. Med., (2007) 357:266-281. Vitamin-D deficiency. Holick MF.
8. Osteoporos. Int., (1997) 7:439-443. Prevalence of Vitamin D insufficiency in an adult normal population. Dawson-Hughes B., et al.
9. Clinical Laboratory International (2010) Vol 34: 16-19. Defining deficiency of vitamin-D. Heaney RP.

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