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Angiotensinogen ELISA Kit Maximize

Angiotensinogen ELISA Kit

Human Angiotensinogen ELISA Kit (JP27412) is an in-vitro quantitative protocol designed to detect levels of human angiotensinogen present.

More details

JP27412

96 wells

£ 680.00

Human Angiotensinogen ELISA Kit

Specificity : Human
Sensitivity : 0.03ng/ml
Assay Range : 0.31 – 20ng/ml
Size: 96 tests

Reagents Supplied:
- Precoated plate : Anti-AGT (72) [96 Wells]
- Standard : Human Angiotensinogen [2x 0.5ml]
- Labeled antibody Conc. : (30X) HRP conjugated Anti- AGT (601) [0.4ml]
- EIA buffer [1x 30ml]
- Solution for Labeled antibody [12ml]
- Wash buffer Conc. : (40X) [50ml]
- TMB Chromogen Solution [15ml]
- Stop Solution [12ml]

Key Features:
[1]. Method:  ELISA
[2]. Kit Size: 96 Wells [12x8].
[3]. Standard Range:  0.31 – 20 ng/ml
[4]. Incubation Time: 1 x 1 h , 2 x 30 min
[5]. Substrate: TMB 450nm
[6]. Volume/Specimen: 100μl EDTA plasma, serum, culture medium, urine.
[7].Regulatory Status:  Research Use Only.

Intended Use:
Human Angiotensinogen ELISA kit is to be used for quantitatively measuring concentrations of angiotensinogen in human EDTA plasma, urine, serum and cell supernatant samples.

Specificity [Cross Reactivity %]:
- Human Angiotensinogen  [100%]
- [Below 0.1%] for Angiotensin I-IV, Angiotensin (1-7), Angiotensin (1-9), Human albumin, Human IgG, Human Angiopoietin-like 3.

Background:
In recent years interest related to the role of the renin-angiotensin system in arterial pressure control and the pathophysiology of hypertension has been shifting to its local role in various tissues. Angiotensinogen is the precursor of angiotensin and is cleaved into angiotensin I and II in the renin-angiotensin system, and it has long been reported to play an important role in controlling blood pressure.

References
1. Hypertension (2001) 37 (5):1329-35. Enhancement of angiotensinogen expression in angiotensin II-dependent hypertension. Kobori H., et al.
2. Am J Physiol Renal Physiol. (2007) 293 (3): 956-60. Novel sandwich ELISA for human angiotensinogen. Katsurada A., et al.
3. Pharmacol Rev. (2007) 59 (3): 251-87. The intrarenal renin-angiotensin system: from physiology to the pathobiology of hypertension and kidney disease. Kobori H., et al.
4. J Am Soc Nephrol. (2001) 12 (3): 431-9. Expression of angiotensinogen mRNA and protein in angiotensin II-dependent hypertension. Kobori H., et al.
5. Hypertension. (2004) 43 (5): 1126-32. AT1 receptor mediated augmentation of intrarenal angiotensinogen in angiotensin II-dependent hypertension. Kobori H., et al.

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