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Adiponectin ELISA Kit Maximize

Adiponectin ELISA Kit

Human Adiponectin ELISA Kit (BV51001) is a diagnostic in-vitro method for quantitative analysis of human adiponectin concentrations.

More details

BV51001

96 wells

£ 595.00

Human Adiponectin ELISA Kit

Specificity : Human, plasma, serum
Sensitivity : 0.26ng/ml
Assay Range : 0.1 – 10ng/ml
Size: 96 tests

Reagents Supplied:
- Adiponectin Antigen Coated Microtiter Strips [96 wells]
- Conjugate Solution [7ml]
- Adiponectin Set of Standards conc. [0.22ml/ vial]
- Adiponectin Quality Control HIGH and LOW [2x 0.4ml]
- Dilution Buffer [2 x 13ml]
- Wash Solution Conc. (10x) [100ml]
- Substrate Solution  [2 x 13ml]
- Stop Solution [9ml]
- Data Sheet + Certificate of Analysis [1x]

Key Features:
[1]. Method:  ELISA
[2]. Kit Size: 96 Wells [12x8].
[3]. Standard Range:  0.1 – 10 ng/ml
[4]. Incubation Time: 1 x 2 h , 1 x 15 min
[5]. Substrate: TMB 450nm
[6]. Volume/Specimen: 10μl EDTA plasma, serum.
[7].Regulatory Status:  CE , EU.

Intended Use:
Human adiponectin ELISA kit intended for in vitro quantitatively diagnostic determination of human adiponectin levels in either human plasma or serum samples.

Specificity:
- Human adiponection 100% (both natural or recombinant).
- No cross reactivity for leptin receptor, human leptin and resistin (100 ng/ml).
- No interfererence for bilirubin-mixed isomers (0.4 mg/ml), hemoglobin (5 mg/ml) or triglycerides (0.25 mg/ml).

References
1. Am J Obstet Gynecol. (2006) 194 (1):100-4. Insulin resistance in postmenopausal women with metabolic syndrome and the measurements of adiponectin, leptin, resistin, and ghrelin. Chu MC., et al.
2. Atherosclerosis. 2008 Mar;197 (1):428-34. Relationship between CT anthropometric measurements, adipokines and abdominal aortic calcification. Golledge J., et al.
3. Clin Rheumatol. (2008) 27 (6):795-7. Effect of anti-TNF treatment on body composition and serum adiponectin levels of women with rheumatoid arthritis. Serelis J., et al.
4. J Clin Endocrinol Metab. (2008) 93 (4):1339-44. Differential sensitivity of men and women to anorexigenic and memory-improving effects of intranasal insulin. Benedict C., et al.
5. Eur J Endocrinol. (2008) 158 (4):533-41. Total adiponectin and adiponectin multimeric complexes in relation to weight loss induced improvements in insulin sensitivity in obese women: the NUGENOB study. Polak J., et al.
6. Cytokine . (2006) 35(5-6):247-52. Increased adiponectin is negatively linked to the local inflammatory process in patients with rheumatoid arthritis. Senolt L, Pavelka K, Housa D, Haluzik M.
7. Clin Endocrinol (2008) 68 (2):213-9. Expression of caveolin-1 in human adipose tissue is upregulated in obesity and obesity-associated type 2 diabetes mellitus and related to inflammation. Catalan V., et al.
8. Diabetes . (2005) 54(9):2712-9. Changes of adiponectin oligomer composition by moderate weight reduction. Bobbert A., et al.
9. Acta Diabetol. (2007) 44 (2):65-8. Short-term therapy with atorvastatin or fenofibrate does not affect plasma ghrelin, resistin or adiponectin levels in type 2 diabetic patients with mixed hyperlipoproteinaemia. Otto C., et al.
10. Diabetes . (2006) 55(1):141-7. Adiponectin does not cross the blood-brain barrier but modifies cytokine expression of brain endothelial Cells. Spranger J., et al.
11. J Clin Endocrinol Metab . (2007) 92(9):3719-27. Plasma osteopontin levels and expression in adipose tissue are increased in obesity. Gomez-Ambrosi J., et al.

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