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Angiotensinogen ELISA Kit Maximize

Angiotensinogen ELISA Kit

Rat Angiotensinogen ELISA Kit (JP27414) is a quantitative method intended to detect levels of rat angiotensinogen present.

More details

JP27414

96 wells

£ 680.00

Rat Angiotensinogen ELISA Kit

Specificity : Rat
Sensitivity : 0.01ng/ml
Assay Range : 0.08 – 5ng/ml
Size: 96 tests

Reagents Supplied:
- Pre-coated Anti-Rat AGT coated Microtiterwell Plate [1x 96 wells]
- HRP Anti-Rat AGT Labeled antibody Conc. (30X)  [1x 0.4ml]
- Native Rat Angiotensinogen Standard  [2x 1ml]
- EIA buffer  [1x 30ml]
- Antibody Label Solution  [1x 12ml]
- TMB Chromogen Solution  [1x 15ml]
- Stop Solution  [1x 12ml]
- Wash Buffer Conc. (40X)  [1x 50ml]

Key Features:
[1]. Method:  ELISA.
[2]. Kit Size: 96 Wells [12x8].
[3]. Standard Range:  0.08 – 5 ng/ml.
[4]. Incubation Time: 1 x 1 h , 2 x 30 min.
[5]. Substrate: TMB 450nm.
[6]. Volume/Specimen: 100μl cell supernatant, EDTA-plasma, serum, urine.
[7].Regulatory Status:  Research Use Only.

Intended Use:
Rat Angiotensinogen ELISA Kit is a method for determining quantitatively the levels of measurement of rat angiotensinogen in EDTA-plasma, cell supernatant serum and urine samples.

Specificity [Cross Reactivity %]:
- Angiotensinogen (Rat)[100 %]
- [Below 0.1%] for the following: Angiotensin I-IV, Angiotensin (1-7), Angiotensin (1-9), Rat Albumin, Rat IgG.

Background:
In recent years interest related to the role of the renin-angiotensin system in arterial pressure control and the pathophysiology of hypertension has been shifting to its local role in various tissues. Angiotensinogen is the precursor of angiotensin and is cleaved into angiotensin I and II in the renin-angiotensin system, and it has long been reported to play an important role in controlling blood pressure.

References
1. Hypertension (2001) 37 (5):1329-35. Enhancement of angiotensinogen expression in angiotensin II-dependent hypertension. Kobori H., et al.
2. Pharmacol Rev. (2007) 59 (3): 251-87. The intrarenal renin-angiotensin system: from physiology to the pathobiology of hypertension and kidney disease. Kobori H., et al.
3. Hypertension. (2004) 43 (5): 1126-32. AT1 receptor mediated augmentation of intrarenal angiotensinogen in angiotensin II-dependent hypertension. Kobori H., et al.
4. J Am Soc Nephrol. (2001) 12 (3): 431-9. Expression of angiotensinogen mRNA and protein in angiotensin II-dependent hypertension. Kobori H., et al.
5. Am J Physiol Renal Physiol. (2007) 293 (3): 956-60. Novel sandwich ELISA for human angiotensinogen. Katsurada A., et al.

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