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Adenovirus IgA ELISA Kit Maximize

Adenovirus IgA ELISA Kit

Adenovirus IgA ELISA Kit (DEADV02) is a quantitative method designed to measure specific IgA antibodies against adenovirus-IgA concentrations.

More details

DEADV02

96 wells

£ 250.00

Adenovirus IgA ELISA Kit

Specificity : Serum, Plasma
Sensitivity : 1 U/ml
Assay Range : 1 – 200 U/ml
Size: 96 tests

Reagents Supplied:
- Antigen coated microtiter strips [12x8]
- Calibrator A [2mL]
- Calibrator D [2mL]
- Calibrator B [2mL]
- Calibrator C [2mL]
- Substrate  [15mL]
- Enzyme Conjugate [15mL]
- Sample Diluent  [60mL]
- Stop Solution  [15mL]
- Washing Buffer (10×)  [60 mL]
- Plastic bag  [x1]

Key Features:
[1]. Method:  ELISA
[2]. Kit Size: 96 Wells [12x8].
[3]. Standard Range:  1 – 200 U/ml
[4]. Sample Preparation: 1:101 pre-dilution
[5]. Incubation Time: 60 min, 30 min, 20 min at RT
[6]. Substrate: TMB 450nm

Intended Use:
Adenovirus IgA ELISA is designed for quantitatively detecting  concentrations of specific IgA antibodies against adenovirus-IgA using either serum or plasma samples.

Expected Values:
In an in-house study apparently healthy subjects showed the following results:
IgA (n=51): 8.0% (positive); 1.1% (equivocal) and 90.9% (negative).
* Each lab. is advised to determine its own normal range.

Interferences:
No interferences to:
1. Hemoglobin up to 8.0 mg/mL
2. Bilirubin  up to 0.3 mg/mL,
3. Triglycerides up to 5.0 mg/mL

Clinical Specificity: 98 %

Clinical Sensitivity: 100%.

Background:
Adenoviruses are endemic in all populations throughout the year. The incubation period is between five and seven days. The adenovirus infection is the most frequently caused viral disease of the respiratory tract among preschool children. The adenovirus is an ubiquitous pathogen of humans and animals. The infection is spread both through the aerial-droplet route and the routes characteristic for intestinal infections. Adenoviruses cause the widest variety of illnesses of the known respiratory viruses. Pneumonia is the most severe form of adenoviral infection occurring mostly in infants below the age of one. An acute adenoviral infection can be detected by virus isolation and/or serology. The serologic tests are particularly important because they document actual infection in the patient and can be applied to large-scale epidemiologic investigations. IgG is the predominant antibody class measured in the serologic tests.

References
1. Manual of Clinical Laboratory Immunology (third edition). American Society for Microbiology, Washington D.C. 527 (1986). Hierholzer JC, Tannock GA. Adenoviruses. In: Rose RR, Friedman H, Fahey JL.
2. Intervirology 18: 169 (1982). Adenoviridae: Second report. Wigand R et al.
3. J. Inf. Dis., 164: 1113 (1991). A Community Outbreak of Epidemic Keratoconjunctivitis in Central Australia Due to Adenovirus Type 8. Mc Minn PC, Stewart J, Burrell CJ.
4. Principles and Practice. Springer Verlag Berlin, New York, Heidelberg, London, Paris, Tokyo. Vol II: 284 (1988). Adenoviridae: The adenoviruses. In: Balows, Hausling, Ohasi & Turono. Laboratory Diagnosis of Infectious Diseases.  Wadell G.

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