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Adenovirus IgM ELISA Kit Maximize

Adenovirus IgM ELISA Kit

Adenovirus IgM ELISA Kit (DEADV03) is designed to be used to measure quantitatively the amounts of specific IgM antibodies against adenovirus-IgM.

More details

DEADV03

96 wells

£ 250.00

Adenovirus IgM ELISA Kit

Specificity : Plasma, Serum
Sensitivity : 1 U/ml
Assay Range : 1 – 150 U/ml
Size: 96 tests

Reagents Supplied:
- Adenovirus antigen coated microtiter strips [12x8]
- Calibrator D [2mL]
- Calibrator A [2mL]
- Calibrator C [2mL]
- Calibrator B [2mL]
- Sample Diluent  [60mL]
- Enzyme Conjugate [15mL]
- Substrate  [15mL]
- Washing Buffer (10×)  [60 mL]
- Stop Solution  [15mL]
- Plastic bag  [x1]

Key Features:
[1]. Method:  ELISA
[2]. Kit Size: 96 Wells [12x8].
[3]. Sample Preparation: 1:101 pre-dilution
[4]. Standard Range:  1 – 150 U/ml
[5]. Incubation Time: 60 min, 30 min, 20 min at RT
[6]. Volume/Specimen: 5μl serum or plasma
[7]. Substrate: TMB 450nm

Intended Use:
Adenovirus IgM ELISA is to be used for quantitative analysis of specific IgM antibodies against adenovirus-IgM levels using plasma and serum samples.

Expected Values:
In an in-house study apparently healthy subjects showed the following results:
IgM (n=72): 0.0% (positive); 0.0% (equivocal) and 100.0% (negative).
* Each lab. is advised to determine its own normal range.

Interferences:
No interferences to:
1. Triglycerides up to 5.0 mg/mL
2. Bilirubin  up to 0.3 mg/mL,
3. Hemoglobin up to 8.0 mg/mL

Clinical Specificity: 100 %

Clinical Sensitivity: 100%.

Background:
Adenoviruses are endemic in all populations throughout the year. The incubation period is between five and seven days. The adenovirus infection is the most frequently caused viral disease of the respiratory tract among preschool children. The adenovirus is an ubiquitous pathogen of humans and animals. The infection is spread both through the aerial-droplet route and the routes characteristic for intestinal infections. Adenoviruses cause the widest variety of illnesses of the known respiratory viruses. Pneumonia is the most severe form of adenoviral infection occurring mostly in infants below the age of one. An acute adenoviral infection can be detected by virus isolation and/or serology. The serologic tests are particularly important because they document actual infection in the patient and can be applied to large-scale epidemiologic investigations. IgG is the predominant antibody class measured in the serologic tests.

References
1. Intervirology 18: 169 (1982). Adenoviridae: Second report. Wigand R et al.
2. J. Inf. Dis., 164: 1113 (1991). A Community Outbreak of Epidemic Keratoconjunctivitis in Central Australia Due to Adenovirus Type 8. Mc Minn PC, Stewart J, Burrell CJ.
3. Manual of Clinical Laboratory Immunology (third edition). American Society for Microbiology, Washington D.C. 527 (1986). Hierholzer JC, Tannock GA. Adenoviruses. In: Rose RR, Friedman H, Fahey JL.
4. Principles and Practice. Springer Verlag Berlin, New York, Heidelberg, London, Paris, Tokyo. Vol II: 284 (1988). Adenoviridae: The adenoviruses. In: Balows, Hausling, Ohasi & Turono. Laboratory Diagnosis of Infectious Diseases.  Wadell G.

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