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Aspergillus fumigatus IgA ELISA Kit Maximize

Aspergillus fumigatus IgA ELISA Kit

Aspergillus fumigatus IgA ELISA Kit (DEASP02) is a procedure for the quantitative analysis of specific IgA antibodies against  Aspergillus fumigatus-IgA.

More details

DEASP02

96 wells

£ 250.00

Aspergillus fumigatus IgA ELISA Kit

Specificity : Plasma, Serum
Sensitivity : 1.14 U/ml
Assay Range : 1 –140 U/ml
Size: 96 tests

Reagents Supplied:
- Coated microtiter strips [12x8]
- Calibrator C [2mL]
- Calibrator A [2mL]
- Calibrator D [2mL]
- Calibrator B [2mL]
- Enzyme Conjugate [15mL]
- Sample Diluent  [60mL]
- Washing Buffer (10×)  [60 mL]
- Substrate  [15mL]
- Stop Solution  [15mL]
- Plastic bag  [x1]

Key Features:
[1]. Method:  ELISA
[2]. Kit Size: 96 Wells [12x8].
[3]. Volume/Specimen: 5μl serum and plasma
[4]. Standard Range:  1 – 140 U/ml
[5]. Sample Preparation: 1:101 pre-dilution
[6]. Incubation Time: 60 min, 30 min, 20 min at Room Temp.
[7]. Substrate: TMB 450nm

Intended Use:
This ELISA kit for aspergillus fumigatus IgA is a protocol for quantitatively detecting specific IgA antibodies against Aspergillus fumigatus-IgA using plasma and serum.

Expected Values:
In an in-house study apparently healthy subjects showed the following results:
Aspergillus fumigatus-IgA (n=88): 2.3% (positive); 1.1% (equivocal) and 96.6% (negative).
* Each laboratory is advised to determine its own normal range.

Interferences:
No interferences to:
1. Triglycerides not greater than 5.0 mg/mL
2. Bilirubin not greater than 0.3 mg/mL,
3. Hemoglobin not more than 8.0 mg/mL

Cross Reactivity: No cross-reactivity to Candida albicans.

Clinical Specificity: 99 %

Clinical Sensitivity: 100%.

Background:
The main opportunistic invasive fungial infections are the candidal mycosis followed by aspergillosis. Generally infections with Aspergillus spp. are airborne. Usually infection in man occurs in already damaged tissues only. The most common pathogen of this genus is A. fumingatus which occurs in hay, grain, rotten plants and birds faeces. Aspergillus species of known pathogenicity to man are Aspergillus fumigatus, A. niger, A. flavus, A. nidulans and A. terreus. Aspergillus spp. can cause a chronical infection of paranasal sinus, eyes or lungs. Because of the ubiquity of Aspergillus species it renders more difficult to decide between contamination by commensals or a serious infection.

References
1. Eur. J. Clin Microbiol. Infect., (1989) 8: 413. Bodey GP.
2. Clin. Microbiol. Rev., (1991) 4: 439. Immunodiagnosis of aspergillosis. Kurup VP, Kumar A. 
3. J. Med., (1986) 81: 249. Significance of Aspergillus species isolated from respiratory secretions in the diagnosis of invasive pulmonary aspergillosis. Yu VL, et al.
4. Dtsch. med. Wschr., (1992) 117: 1681. The clinical picture and diagnosis of pulmonary mycoses. Schaberg T, Mauch H, Lode H.
5. Ann. intern. Med., (1977) 86: 405. Clinical and immunologic criteria for the diagnosis of allergic bronchopulmonary aspergillosis. Rosenberg M, Pattersom R, Mintzer R, Cooper BJ, Roberts M, Harris KE.
5. Arch. intern. Med., (1986) 146: 986. Allergic bronchopulmonary aspergillosis. Natural history and classification of early disease by serologic and roentgenographic studies. Patterson R, Greenberger PA, Halwig JM, Liotta L, Roberts M.
7.  Dtsch. med. Wschr., (1989) 114: 1706. Boeckh M, Höffgen G, Lode H.
8. Annals of Hematology (1993) 67: 1. Diagnosis of invasive mycoses in severely immunosuppressed patients. Rüchel R.

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