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Aspergillus fumigatus IgM ELISA Kit Maximize

Aspergillus fumigatus IgM ELISA Kit

Aspergillus fumigatus IgM ELISA Kit (DEASP03) is a quantitative protocol intended to determine amounts of specific IgM antibodies against  Aspergillus fumigatus-IgM present.

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DEASP03

96 wells

£ 250.00

Aspergillus fumigatus IgM ELISA Kit

Specificity : Plasma, Serum
Sensitivity : 1.04 U/ml
Assay Range : 1 –60 U/ml
Size: 96 tests

Reagents Supplied:
- Coated microtiter strips [12x8]
- Calibrator A [2mL]
- Calibrator D [2mL]
- Calibrator C [2mL]
- Calibrator B [2mL]
- Enzyme Conjugate [15mL]
- Washing Buffer (10×)  [60 mL]
- Sample Diluent  [60mL]
- Substrate  [15mL]
- Stop Solution  [15mL]
- Plastic bag  [x1]

Key Features:
[1]. Method:  ELISA
[2]. Kit Size: 96 Wells [12x8].
[3]. Volume/Specimen: 5μl serum or plasma
[4]. Standard Range:  1 – 60 U/ml
[5]. Sample Preparation: 1:101 pre-dilution
[6]. Incubation Time: 60 min, 30 min, 20 min at Room Temperature.
[7]. Substrate: TMB 450nm

Intended Use:
Aspergillus fumigatus IgM ELISA is designed for quantitatively measuring concentrations of specific IgM antibodies against Aspergillus fumigatus-IgM with serum and plasma.

Expected Values:
In an in-house study apparently healthy subjects showed the following results:
Aspergillus fumigatus-IgM (n=88): 2.3% (positive); 4.6% (equivocal) and 93.2% (negative).
* Each laboratory is advised to determine its own normal range.

Interferences:
No interferences to:
1. Bilirubin not greater than 0.3 mg/mL,
2. Triglycerides not greater than 5.0 mg/mL
3. Hemoglobin not more than 8.0 mg/mL

Cross Reactivity: No cross-reactivity to Candida albicans.

Clinical Specificity: 99 %

Clinical Sensitivity: 100%.

Background:
The main opportunistic invasive fungial infections are the candidal mycosis followed by aspergillosis. Generally infections with Aspergillus spp. are airborne. Usually infection in man occurs in already damaged tissues only. The most common pathogen of this genus is A. fumingatus which occurs in hay, grain, rotten plants and birds faeces. Aspergillus species of known pathogenicity to man are Aspergillus fumigatus, A. niger, A. flavus, A. nidulans and A. terreus. Aspergillus spp. can cause a chronical infection of paranasal sinus, eyes or lungs. Because of the ubiquity of Aspergillus species it renders more difficult to decide between contamination by commensals or a serious infection.

References
1. Eur. J. Clin Microbiol. Infect., (1989) 8: 413. Bodey GP.
2. Clin. Microbiol. Rev., (1991) 4: 439. Immunodiagnosis of aspergillosis. Kurup VP, Kumar A. 
3. J. Med., (1986) 81: 249. Significance of Aspergillus species isolated from respiratory secretions in the diagnosis of invasive pulmonary aspergillosis. Yu VL, et al.
4. Dtsch. med. Wschr., (1992) 117: 1681. The clinical picture and diagnosis of pulmonary mycoses. Schaberg T, Mauch H, Lode H.
5. Ann. intern. Med., (1977) 86: 405. Clinical and immunologic criteria for the diagnosis of allergic bronchopulmonary aspergillosis. Rosenberg M, Pattersom R, Mintzer R, Cooper BJ, Roberts M, Harris KE.
5. Arch. intern. Med., (1986) 146: 986. Allergic bronchopulmonary aspergillosis. Natural history and classification of early disease by serologic and roentgenographic studies. Patterson R, Greenberger PA, Halwig JM, Liotta L, Roberts M.
7.  Dtsch. med. Wschr., (1989) 114: 1706. Boeckh M, Höffgen G, Lode H.
8. Annals of Hematology (1993) 67: 1. Diagnosis of invasive mycoses in severely immunosuppressed patients. Rüchel R.

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