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ACTH Fluorescent EIA Kit Maximize

ACTH Fluorescent EIA Kit

Human ACTH Fluorescent EIA Kit (FEK-001-01) can be used to measure quantitative in-vitro amounts of human adrenocorticotropic hormone (ACTH).

More details

FEK-001-01

96 wells

£ 480.00

Human ACTH Fluorescent EIA Kit

Specificity : Human
Sensitivity : 42.7 pg/ml
Linear Range: 42.7-1828 pg/ml
Range: 0-10000 pg/ml
Size: 96 tests

Reagents Supplied:
- 96 well immunoplate (1 plate)
- 20x assay buffer concentrate (50ml)
- Standard peptide (1 vial).
- Primary antibody (rabbit anti-peptide IgG) (1 vial).
- Positive control (1 vial).
- Acetate plate sealer (APS), (3 pieces)
- Streptavidin-horseradish peroxidase (SA-HRP) (30μl)
- Substrate solution (12ml)
- Biotinylated peptide (1 vial).
- Stable peroxide solution (1.5ml)
- Stop solution (12ml)
- General protocol (1 book).
- Assay diagram (1 sheet).

Intended Use:
Human ACTH Fluorescent EIA Kit is intended for quantitative in-vitro determination of human adrenocorticotropic hormone (ACTH) levels in plasma, serum, culture media, tissue homogenate, CSF, urine or any biological fluid samples.

Assay Principle:
1. Human ACTH Fluorescent EIA has been pre-coated with secondary antibody and also the non-specific binding sites have been blocked.
2. Secondary antibody is able to bind with the Fc fragment of the primary antibody, the Fab segment is competitively bound by both biotinylated peptides and the peptide standard or the targeted peptide in samples.
3. Biotinylated peptide can then interact with the SA-HRP, which then catalyzes the substrate solution.
4. Fluorescence intensity is directly proportional to the amount of biotinylated peptide-SA-HRP complex but inversely proportional to the amount of the peptide in standard solutions or samples.
5. Unknown concentration in samples can then be determined by extrapolation from a standard curve.

Cross Reactivity:
ACTH (Human) 100%.
ACTH (Rat) 100%.
ACTH (1-24) (Human) 100%.
ACTH (7-38) (Human) 100%.
Beta-Endorphin (Human) 0%.
CRF (Human, Rat) 0%,
Alpha-MSH 0%.
Met-Enkephalin 0%.
Alpha-ANP (1-28) (Human, Canine) 0%.
BNP-32 (Human) 0%.

Background:
Adrenocorticotropic hormone (ACTH or corticotropin) is a polypeptide hormone produced and secreted by the pituitary gland in response to the release of another hormone, called corticotropin-releasing hormone (CRH), by the hypothalamus. It is found to act through the stimulation of cell surface. It is made up of 39 amino acids, the first 13 of which may be cleaved to form α-melanocyte-stimulating hormone (α-MSH). Receptors are primarily located on the adrenocortical cells. Released in bursts, so its levels in the blood can vary from minute to minute. It is secreted in several intermittent pulses during the day into the bloodstream and transported around the body. When cortisol levels rise, ACTH levels normally fall.  When cortisol levels fall, ACTH levels normally rise. It also increases production of the chemical compounds that trigger an increase in other hormones such as adrenaline and noradrenaline.

References
1. Traffic. (2007) 8 (7): 867-82. Rab18 Inhibits Secretory Activity in Neuroendocrine Cells by Interacting with Secretory Granules. Vazquez-Martinez et al.
2. J Gastroenterol. (2007) 42 (7): 522-7. Stress maladjustment in the pathoetiology of ulcerative colitis. Kuroki et al.
3. Br J Dermatol. (2006) 155 (5): 910-5. Investigation of the corticotropin-releasing hormone-proopiomelanocortin axis in various skin tumours. Kim et al.
4. Ann N Y Acad Sci. (1999) 885: 268-76. The Role of Proopiomelanocortin-Derived Peptides in Skin Fibroblast and Mast Cell Functions. Teofoli et al.

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